Supplier: BioVision
Catalogue number: K930-100
The Cell Stimulation Cocktail (500X) is a ready-to-use cocktail useful to induce activation of many cell types to produce cytokines. This cocktail can be used to induce cytokine production in in vitro cell culture for subsequent detection in immunoassays. Each vial contains Protein Kinase C activator Phorbol 12-Myristate 13-Acetate (PMA) (40.5 µM) and the calcium ionophore, Ionomycin (670 µM) formulated in DMSO (500X).
Product Details
biovision ezcell cocktail
Description
The Cell Stimulation and Protein Transport Inhibitor Cocktail is a ready-to-use cocktail consisting of an optimized mixture of Phorbol 12-Myristate 13-Acetate (PMA), Ionomycin, Brefeldin A, and monesin. Formulated in DMSO (500X). PMA and Ionomycin in duce cytokine production in many cell types and Brefeldin A and Monensin act as protein transport inhibitors. This cocktail is used to induce cytokine production and inhibit their intracellular transport for flow cytometry detection.
The EZCell Protein Transport Inhibitor Cocktail (500X) is a cocktail composed of of Brefeldin A and Monensin, formulated in DMSO. Useful in assays that require prevention of protein transport to the extracellular space is required. Addition of this cocktail to cells in culture inhibits the intracellular protein secretory/transport pathway resulting in the accumulation of secreted proteins in the lumen of the endoplasmic reticulum and in the Golgi apparatus. This accumulation can then be detected by intracellular staining and flow cytometric analysis or Western blotting.
More Information
SKU
K932-500
Size
500 ul
Application
A Protein Transport Inhibitor cocktail
Cas no
See under individual products
Storage
-20C
Shipping Temp
Gel Pack
Carboxyfluorescein Diacetate Succinimidyl Ester (CFDA SE) is a colorless, non-fluorescent, stable cell-permeable dye. Once it enters into the cells, the intracellular esterases remove the acetate groups to generate Carboxyfluorescein Succinimidyl Ester, which then covalently binds to lysine residues or other amines and produces a bright fluorescent conjugate. The fluorescent conjugate is stable and is retained inside the cells during cell division and hence it is an ideal tool for cell tracing studies, both in vitro and in vivo. The EZCell™ CFDA SE Cell Tracer Kit provides an easy, rapid and sensitive way to determine cell proliferation using CFDA SE dye. The dye can be inherited by the daughter cells for up to 8 generations. Following each cell division, the fluorescence intensity is halved, which can be quantified by measuring the fluorescence intensity at Ex/Em = 492/517 nm. The assay is non-radioactive, accurate and can be used for cell tracing studies.
Alternate Name : EZCell™ Cell Tracer Kit,CFDA Cell Tracer Kit,CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Kit,Cell Tracer Kit
Tag Line : Flow cytometry based assay to determine cell proliferation using CFDA SE dye
Summary : BioVisions EZCell™ CFDA SE Cell Tracer Kit provides an easy, rapid and sensitive way to determine cell proliferation using CFDA SE dye. The dye can be inherited by the daughter cells for up to 8 generations. Following each cell division, the fluorescence intensity is halved, which can be quantified by measuring the fluorescence intensity at Ex/Em = 492/517 nm. The assay is non-radioactive, accurate and can be used for cell tracing studies.
Cell Stimulation Cocktail (500X) 100 uL
E16FXP048-100
100 μl
EUR 193
Description: Available in various conjugation types.
Cell Stimulation Cocktail (500X) 4 x 100 uL
E16FXP048-4x100
4x100 μl
EUR 559.33
Description: Available in various conjugation types.
Cell Stimulation and Protein Transport Inhibitor Cocktail
MBS697185-01mL
0.1mL
EUR 195
Cell Stimulation and Protein Transport Inhibitor Cocktail
MBS697185-5x01mL
5x0.1mL
EUR 825
EZCell? Calcium Detection Kit (Cell-Based)
K432-50
each
EUR 679.2
EZCell? Cell Invasion Assay Kit (Laminin), 12-well, 8 µm
K915-12
each
EUR 614.4
EZCell? Cell Invasion Assay (Collagen I), 96-well, 8 µm
K916-100
each
EUR 692.4
EZCell? Cell Invasion Assay (Collagen IV), 96-well, 8 µm
K918-100
each
EUR 679.2
EZCell? Cell Migration/Chemotaxis Assay Kit (96-well, 8 µm)
K906-100
each
EUR 679.2
EZCell? Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm)
K907-100
each
EUR 666
EZCell? Cell Migration/Chemotaxis Assay Kit (24-well, 8 µm)
K909-12
each
EUR 548.4
EZCell? Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm)
K910-12
each
EUR 535.2
Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) 100 uL
E16FXP049-100
100 μl
EUR 323.17
Description: Available in various conjugation types.
EZCell? Intracellular Zinc Staining Kit (Cell-Based)
K525-50
each
EUR 679.2
Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) 4 x 100 uL
E16FXP049-4x100
4x100 μl
EUR 849
Description: Available in various conjugation types.
cleavage stimulation factor Antibody
35503
100ul
EUR 479
cleavage stimulation factor Antibody
35503-100ul
100ul
EUR 468
anti-Cleavage stimulation factor 2
YF-PA11164
50 ul
EUR 435.6
Description: Mouse polyclonal to Cleavage stimulation factor 2
anti-Cleavage stimulation factor 2
YF-PA11165
50 ug
EUR 435.6
Description: Mouse polyclonal to Cleavage stimulation factor 2
anti-Cleavage stimulation factor 2
YF-PA11166
100 ul
EUR 483.6
Description: Rabbit polyclonal to Cleavage stimulation factor 2
anti-Cleavage stimulation factor 2
YF-PA11167
100 ug
EUR 483.6
Description: Rabbit polyclonal to Cleavage stimulation factor 2
anti-Cleavage stimulation factor 2
YF-PA23534
50 ul
EUR 400.8
Description: Mouse polyclonal to Cleavage stimulation factor 2
anti-Cleavage stimulation factor 3
YF-PA23535
50 ul
EUR 400.8
Description: Mouse polyclonal to Cleavage stimulation factor 3
Cleavage Stimulation Factor 1 Protein
20-abx260926
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Anti-cleavage stimulation factor (1C6)
YF-MA12593
100 ug
EUR 435.6
Description: Mouse monoclonal to cleavage stimulation factor
Cleavage Stimulation Factor 1 Protein
abx260926-10g
10 µg
EUR 325
Cleavage Stimulation Factor 1 Protein
abx260926-2g
2 µg
EUR 225
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit)
MBS6013081-02mL
0.2(mL
EUR 695
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit)
MBS6013081-5x02mL
5x0.2mL
EUR 2975
Growth Stimulation Expressed Gene 2 (ST2)
E35K2966
100ug
EUR 295
Anti-Cleavage stimulation factor 2 (1D4)
YF-MA10205
100 ug
EUR 435.6
Description: Mouse monoclonal to Cleavage stimulation factor 2
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (AP)
MBS6289528-02mL
0.2mL
EUR 980
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (AP)
MBS6289528-5x02mL
5x0.2mL
EUR 4250
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (APC)
MBS6289529-02mL
0.2mL
EUR 980
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (APC)
MBS6289529-5x02mL
5x0.2mL
EUR 4250
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (FITC)
MBS6289531-02mL
0.2mL
EUR 980
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (FITC)
MBS6289531-5x02mL
5x0.2mL
EUR 4250
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (PE)
MBS6289538-02mL
0.2mL
EUR 980
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (PE)
MBS6289538-5x02mL
5x0.2mL
EUR 4250
TSH (Thyroid Stimulation Hormone) (MaxLight 405)
MBS6450436-01mL
0.1mL
EUR 745
TSH (Thyroid Stimulation Hormone) (MaxLight 490)
MBS6450437-01mL
0.1mL
EUR 745
TSH (Thyroid Stimulation Hormone) (MaxLight 550)
MBS6450438-01mL
0.1mL
EUR 745
TSH (Thyroid Stimulation Hormone) (MaxLight 650)
MBS6450439-01mL
0.1mL
EUR 745
TSH (Thyroid Stimulation Hormone) (MaxLight 750)
MBS6450440-01mL
0.1mL
EUR 745
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (Biotin)
MBS6289530-02mL
0.2mL
EUR 980
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (Biotin)
MBS6289530-5x02mL
5x0.2mL
EUR 4250
Cleavage Stimulation Factor 1 (CSTF1) Antibody
20-abx137481
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CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (MaxLight 405)
MBS6289533-01mL
0.1mL
EUR 980
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (MaxLight 405)
MBS6289533-5x01mL
5x0.1mL
EUR 4250
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (MaxLight 490)
MBS6289534-01mL
0.1mL
EUR 980
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (MaxLight 490)
MBS6289534-5x01mL
5x0.1mL
EUR 4250
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (MaxLight 550)
MBS6289535-01mL
0.1mL
EUR 980
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (MaxLight 550)
MBS6289535-5x01mL
5x0.1mL
EUR 4250
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (MaxLight 650)
MBS6289536-01mL
0.1mL
EUR 980
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (MaxLight 650)
MBS6289536-5x01mL
5x0.1mL
EUR 4250
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (MaxLight 750)
MBS6289537-01mL
0.1mL
EUR 980
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (MaxLight 750)
MBS6289537-5x01mL
5x0.1mL
EUR 4250
Thyroid Stimulation Hormone (TSH) Human, Antibody
GWB-ED882C
1 mg
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Cleavage stimulation factor subunit 1 Antibody
MBS5400372-01mg
0.1mg
EUR 445
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) APC
MBS6136087-01mL
0.1(mL
EUR 875
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) APC
MBS6136087-5x01mL
5x0.1mL
EUR 3800
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (AP)
MBS6130784-01mL
0.1(mL
EUR 875
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (AP)
MBS6130784-5x01mL
5x0.1mL
EUR 3800
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (AP)
MBS6374940-01mL
0.1mL
EUR 920
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (AP)
MBS6374940-5x01mL
5x0.1mL
EUR 3990
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) APC
MBS6374941-01mL
0.1mL
EUR 920
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) APC
MBS6374941-5x01mL
5x0.1mL
EUR 3990
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (FITC)
MBS6374943-01mL
0.1mL
EUR 920
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (FITC)
MBS6374943-5x01mL
5x0.1mL
EUR 3990
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (HRP)
MBS6374944-01mL
0.1mL
EUR 920
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (HRP)
MBS6374944-5x01mL
5x0.1mL
EUR 3990
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (PE)
MBS6374950-01mL
0.1mL
EUR 920
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (PE)
MBS6374950-5x01mL
5x0.1mL
EUR 3990
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (FITC)
MBS6146693-01mL
0.1(mL
EUR 875
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (FITC)
MBS6146693-5x01mL
5x0.1mL
EUR 3800
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (PE)
MBS6157299-01mL
0.1(mL
EUR 875
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (PE)
MBS6157299-5x01mL
5x0.1mL
EUR 3800
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (HRP)
MBS6151996-01mL
0.1(mL
EUR 875
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (HRP)
MBS6151996-5x01mL
5x0.1mL
EUR 3800
Cleavage Stimulation Factor 2, NT (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64, CSTF2)
MBS626293-02mL
0.2mL
EUR 695
Cleavage Stimulation Factor 2, NT (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64, CSTF2)
MBS626293-5x02mL
5x0.2mL
EUR 2975
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (Azide free) (HRP)
MBS6289532-02mL
0.2mL
EUR 980
CSTF3, CT (CSTF3, Cleavage stimulation factor subunit 3, CF-1 77kD subunit, Cleavage stimulation factor 77kD subunit) (Azide free) (HRP)
MBS6289532-5x02mL
5x0.2mL
EUR 4250
Cleavage stimulation factor 2 (CSTF2) Mouse mAb
MBS435177-002mL
0.02mL
EUR 270
Cleavage stimulation factor 2 (CSTF2) Mouse mAb
MBS435177-005mL
0.05mL
EUR 415
Cleavage stimulation factor 2 (CSTF2) Mouse mAb
MBS435177-01mL
0.1mL
EUR 660
Cleavage stimulation factor 2 (CSTF2) Mouse mAb
MBS435177-5x01mL
5x0.1mL
EUR 2655
Cleavage Stimulation Factor 1 Human Recombinant
rAP-3818
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Recombinant Human Cleavage Stimulation Factor 1
MBS144898-0005mg
0.005mg
EUR 240
Recombinant Human Cleavage Stimulation Factor 1
MBS144898-002mg
0.02mg
EUR 310
Recombinant Human Cleavage Stimulation Factor 1
MBS144898-1mg
1mg
EUR 2880
Recombinant Human Cleavage Stimulation Factor 1
MBS144898-5x1mg
5x1mg
EUR 12630
Anti-Cleavage stimulation factor 2 (3D1-3A6)
YF-MA10204
100 ug
EUR 435.6
Description: Mouse monoclonal to Cleavage stimulation factor 2
Anti-Cleavage stimulation factor 2 (4E3-3C9)
YF-MA12594
100 ug
EUR 435.6
Description: Mouse monoclonal to Cleavage stimulation factor 2
Mouse Anti Human Cleavage Stimulation Factor 1
MBS140477-002mg
0.02mg
EUR 310
Mouse Anti Human Cleavage Stimulation Factor 1
MBS140477-01mg
0.1mg
EUR 610
Mouse Anti Human Cleavage Stimulation Factor 1
MBS140477-5x01mg
5x0.1mg
EUR 2405
Cleavage Stimulation Factor 2, NT (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64, CSTF2) (AP)
MBS6470755-02mL
0.2mL
EUR 980
Cleavage Stimulation Factor 2, NT (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64, CSTF2) (AP)
MBS6470755-5x02mL
5x0.2mL
EUR 4250
Cleavage Stimulation Factor 2, NT (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64, CSTF2) (APC)
MBS6470756-02mL
0.2mL
EUR 980
Cleavage Stimulation Factor 2, NT (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64, CSTF2) (APC)
MBS6470756-5x02mL
5x0.2mL
EUR 4250
Cleavage Stimulation Factor 2, NT (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64, CSTF2) (FITC)
MBS6470758-02mL
0.2mL
EUR 980
Cleavage Stimulation Factor 2, NT (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64, CSTF2) (FITC)
MBS6470758-5x02mL
5x0.2mL
EUR 4250
Cleavage Stimulation Factor 2, NT (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64, CSTF2) (PE)
MBS6470765-02mL
0.2mL
EUR 980
Cleavage Stimulation Factor 2, NT (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64, CSTF2) (PE)
MBS6470765-5x02mL
5x0.2mL
EUR 4250
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (Biotin)
MBS6141390-01mL
0.1(mL
EUR 875
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (Biotin)
MBS6141390-5x01mL
5x0.1mL
EUR 3800
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (Biotin)
MBS6374942-01mL
0.1mL
EUR 920
CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (Biotin)
MBS6374942-5x01mL
5x0.1mL
EUR 3990