Supplier: BioVision

Catalogue number: K930-100
Size: 100 µl

 

The Cell Stimulation Cocktail (500X) is a ready-to-use cocktail useful to induce activation of many cell types to produce cytokines. This cocktail can be used to induce cytokine production in in vitro cell culture for subsequent detection in immunoassays. Each vial contains Protein Kinase C activator Phorbol 12-Myristate 13-Acetate (PMA) (40.5 µM) and the calcium ionophore, Ionomycin (670 µM) formulated in DMSO (500X).

Product Details

Appearance Liquid
Formulation In DMSO
CAS # See under individual products
Molecular Formula See under individual products
Molecular Weight See under individual products
Handling Protect from air and light
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.
biovision ezcell cocktail
biovision ezcell cocktail
Description
The Cell Stimulation and Protein Transport Inhibitor Cocktail is a ready-to-use cocktail consisting of an optimized mixture of Phorbol 12-Myristate 13-Acetate (PMA), Ionomycin, Brefeldin A, and monesin. Formulated in DMSO (500X). PMA and Ionomycin in duce cytokine production in many cell types and Brefeldin A and Monensin act as protein transport inhibitors. This cocktail is used to induce cytokine production and inhibit their intracellular transport for flow cytometry detection.
The EZCell Protein Transport Inhibitor Cocktail (500X) is a cocktail composed of of Brefeldin A and Monensin, formulated in DMSO. Useful in assays that require prevention of protein transport to the extracellular space is required. Addition of this cocktail to cells in culture inhibits the intracellular protein secretory/transport pathway resulting in the accumulation of secreted proteins in the lumen of the endoplasmic reticulum and in the Golgi apparatus. This accumulation can then be detected by intracellular staining and flow cytometric analysis or Western blotting.
More Information
SKU K932-500
Size 500 ul
Application A Protein Transport Inhibitor cocktail
Cas no See under individual products
Storage -20C
Shipping Temp Gel Pack

Carboxyfluorescein Diacetate Succinimidyl Ester (CFDA SE) is a colorless, non-fluorescent, stable cell-permeable dye. Once it enters into the cells, the intracellular esterases remove the acetate groups to generate Carboxyfluorescein Succinimidyl Ester, which then covalently binds to lysine residues or other amines and produces a bright fluorescent conjugate. The fluorescent conjugate is stable and is retained inside the cells during cell division and hence it is an ideal tool for cell tracing studies, both in vitro and in vivo. The EZCell™ CFDA SE Cell Tracer Kit provides an easy, rapid and sensitive way to determine cell proliferation using CFDA SE dye. The dye can be inherited by the daughter cells for up to 8 generations. Following each cell division, the fluorescence intensity is halved, which can be quantified by measuring the fluorescence intensity at Ex/Em = 492/517 nm. The assay is non-radioactive, accurate and can be used for cell tracing studies.

Alternate Name : EZCell™ Cell Tracer Kit,CFDA Cell Tracer Kit,CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Kit,Cell Tracer Kit

Tag Line : Flow cytometry based assay to determine cell proliferation using CFDA SE dye

Summary : BioVision’s EZCell™ CFDA SE Cell Tracer Kit provides an easy, rapid and sensitive way to determine cell proliferation using CFDA SE dye. The dye can be inherited by the daughter cells for up to 8 generations. Following each cell division, the fluorescence intensity is halved, which can be quantified by measuring the fluorescence intensity at Ex/Em = 492/517 nm. The assay is non-radioactive, accurate and can be used for cell tracing studies.

cleavage stimulation factor Antibody

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EZCell? Direct Glucose Uptake Assay Kit

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EZCell? Protein Transport Inhibitor Cocktail (500X)

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EZCell? Phagocytosis Assay Kit (Green Zymosan)

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EZCell? Phagocytosis Assay Kit (Red Zymosan)

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EZCell? Cell Migration/Chemotaxis Assay Kit (96-well, 8 µm)

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EZCell? Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm)

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EZCell? Cell Invasion Assay (Collagen I), 96-well, 8 µm

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EZCell? Cell Invasion Assay (Collagen IV), 96-well, 8 µm

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Cleavage Stimulation Factor 1 Protein

20-abx260926
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Anti-cleavage stimulation factor (1C6)

YF-MA12593 100 ug
EUR 363
Description: Mouse monoclonal to cleavage stimulation factor

anti-Cleavage stimulation factor 2

YF-PA11164 50 ul
EUR 363
Description: Mouse polyclonal to Cleavage stimulation factor 2

anti-Cleavage stimulation factor 2

YF-PA11165 50 ug
EUR 363
Description: Mouse polyclonal to Cleavage stimulation factor 2

anti-Cleavage stimulation factor 2

YF-PA11166 100 ul
EUR 403
Description: Rabbit polyclonal to Cleavage stimulation factor 2

anti-Cleavage stimulation factor 2

YF-PA11167 100 ug
EUR 403
Description: Rabbit polyclonal to Cleavage stimulation factor 2

anti-Cleavage stimulation factor 2

YF-PA23534 50 ul
EUR 334
Description: Mouse polyclonal to Cleavage stimulation factor 2

anti-Cleavage stimulation factor 3

YF-PA23535 50 ul
EUR 334
Description: Mouse polyclonal to Cleavage stimulation factor 3

EZCell? Phagocytosis Assay Kit (Green E. coli)

K963-100
EUR 430

EZCell? Phagocytosis Assay Kit (Red E. coli)

K964-100
EUR 419

Cleavage Stimulation Factor 1 (CSTF1) Antibody

20-abx137481
  • EUR 704.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 20 ug
  • 5 ug

Anti-Cleavage stimulation factor 2 (1D4)

YF-MA10205 100 ug
EUR 363
Description: Mouse monoclonal to Cleavage stimulation factor 2

EZCell? Intracellular Nitric Oxide Synthase (NOS) Detection Kit

K207-100
EUR 387

Cleavage Stimulation Factor Subunit 2 (CSTF2) Antibody

20-abx006066
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Cleavage Stimulation Factor Subunit 3 (CSTF3) Antibody

abx027771-400ul 400 ul
EUR 523

Cleavage Stimulation Factor Subunit 3 (CSTF3) Antibody

abx027771-80l 80 µl
EUR 286

Cleavage Stimulation Factor Subunit 1 (CSTF1) Antibody

20-abx004532
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Cleavage Stimulation Factor Subunit 3 (CSTF3) Antibody

20-abx124031
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul

Cleavage Stimulation Factor Subunit 3 (CSTF3) Antibody

20-abx125725
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Cleavage Stimulation Factor Subunit 1 (CSTF1) Antibody

abx145028-100ug 100 ug
EUR 391

Cleavage Stimulation Factor Subunit 2 (CSTF2) Antibody

20-abx013470
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Cleavage Stimulation Factor Subunit 1 (CSTF1) Antibody

20-abx013580
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Cleavage Stimulation Factor Subunit 2 (CSTF2) Antibody

20-abx321619
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul

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