The European Collection of Authenticated Cell Cultures (ECACC) was established in 1984 as a cell
culture collection to service the research community and provide an International Depository Authority
recognised patent depository for Europe. Over the last three decades ECACC has expanded and diversified to become one of the premier collections of authenticated cell cultures in the world and this remains the core of ECACC’s business. The collections currently hold over 40,000 cell lines representing 45 different species, 50 tissue types, 300 HLA types, 450 monoclonal antibodies and at least 800 genetic disorders.
The development and maintenance of such a diverse collection has inevitably produced a high level of
specialist knowledge and this has enabled ECACC to position itself as a centre of expertise in all aspects
of cell culture. ECACC has developed a comprehensive range of cell culture services and diversified into
new product areas such as extracted from cell lines.

ECACC is the largest of four collections of clinically significant organisms, maintained by Public Health
England (PHE). The other collections are the National Collection of Type Cultures (NCTC), the National
Collection of Pathogenic Viruses (NCPV), and the National Collection of Pathogenic Fungi (NCPF).

 

Design and Equipment for the Cell Culture Laboratory 2.1 Laboratory Design

Perhaps one of the most undervalued aspects of tissue culture is the need
to design the facility to ensure that good quality material is produced in a
safe and efficient manner. Most tissue culture is undertaken in laboratories
that have been adapted for the purpose and in conditions that are not ideal.
However, as long as a few basic guidelines are adopted this should not
compromise the work.

Cell Culture
Cell Culture

Traditional methods of central nervous system culture

Cell and tissue culture techniques have been central to neuroscience research and the advancing knowledge of key mechanisms underpinning central nervous system (CNS) development, function and malfunction. These methods have permitted investigation of cellular level functions that would be infeasible to carry out routinely in vivo. Not only are animal studies often more time consuming and expensive, the complexity of the in vivo environment can make it difficult to isolate and control confounding variables in order to investigate specific cell and molecular features. In some cases inaccessibility of relevant tissue renders such studies completely impossible.

One of the earliest reports of in vitro CNS cell culture was carried out by Harrison, who observed neurite extension in fragments of frog medullary tube cultured using the hanging drop method, which involves the inversion of tissue-containing solution that has been placed on a cover slip to create a hanging drop [1]. The study was pivotal in representing one of the first examples of CNS tissue being grown ex vivo. By the mid-20th century, human CNS tissue culture had become more common and was often performed in either hanging drop or roller tube formats [2,3]. Studies carried out using roller tube methods involved growing neural tissue explants on rotating surfaces to allow alternating exposure to culture medium and gaseous phases.

In 1955, Cavanaugh became the first to report the application of monolayer culture using neurons derived from chick embryos and then, two decades later by the mid-1970s, monolayer CNS tissue culture had begun to gain popularity. The monolayer cell culture technique relied on dissociation of brain tissues using trypsin or collagenase to form single cell suspensions which were then grown on various glass or plastic substrates. These studies were used to identify morphological, molecular and electrophysiological features of neurons and other CNS relevant cell types in vitro (reviewed in).

Conventional monolayer culture methods have also been referred to as two-dimensional (2D) because they require cell adherence to a flat surface, to which culture medium is applied allowing access to nutrients that enable cell survival and proliferation. While healthy cells remain attached to the surface, necrotic cells become detached and are easily removed during media change. Monolayer tissue culture has since been widely adopted as a routine method for assaying cell activity. Although glass was initially the predominant culture substrate of choice, clear polystyrene has taken precedence due to its cost efficiency, ease of visual analysis, and ability to promote cell adherence without the use of coatings..

 

Cell Culture Plate

CCP01000-00-CellCulturePlate6Well2CellCulturePlate96Well2 Cell Culture Plate,6 Well*2Cell Culture Plate,96 Well*2
EUR 1.09

Water for Cell Culture

TBS5050 500ml
EUR 10

T225 Cell Culture Flask

P0230 25/case
EUR 185

Cell Culture Grade Water

25-511 6 x 500 mL/Unit
EUR 52.91
Description: Cell Culture Tested, Sterile

Cell Culture Grade Water

25-511B 6 x 1000 mL
EUR 85.86
Description: Cell Culture Tested, Sterile

Water, Cell Culture Grade

CA018-600 6x1000ml
EUR 148.8

Hepes Cell Culture Grade

AK3268-0250 250g Ask for price

Hepes Cell Culture Grade

AK3268-0500 500g Ask for price

Hepes Cell Culture Grade

AK3268-1000 1kg Ask for price

CELL CULTURE GRADE WATER

IB42010 1L
EUR 41.13
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42011 6x1L
EUR 239.38
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42012 12x1L
EUR 469.02
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42020 2L
EUR 59.67
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42021 6x2L
EUR 342.87
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42030 10L
EUR 194.72
Description: JUG W/HANDL 0.1 MICRON STERILE

CRFK CELL TUBE CULTURE

63-0600 each Ask for price

Human MEP Cell Culture Medium (human megakaryocyte–erythroid progenitor cell culture medium)

P770 8 rxn
EUR 499

25 cm2 Cell Culture Flask

CCF00025-00-10Bag 10 / Bag
EUR 1.09

25 cm2 Cell Culture Flask

CCF00025-200Case 200 / Case
EUR 186.39

75 cm2 Cell Culture Flask

CCF00075-00-5Bag 5 / Bag
EUR 1.09

75 cm2 Cell Culture Flask

CCF00075-100Case 100 / Case
EUR 176.04

ROCKER CELL CULTURE BAG, 2L

91-200-80 1/pk
EUR 450
Description: Flexible Containers; Bioprocess Bags and Parts

175 cm2 Cell Culture Flask

CCF00175-40Case 40 / Case
EUR 119.36

225 cm2 Cell Culture Flask

CCF00225-25Case 25 / Case
EUR 93.2

Cell Culture Plate, 6 Well

CCP01006-50Case 50 / Case
EUR 61.59

Cell Culture Plate, 96 Well

CCP01096-100Case 100 / Case
EUR 154.24

Ethanolamine, Cell Culture tested

CA071-010 100ml
EUR 116.4

ROCKER CELL CULTURE BAG, 20L

91-200-78 1/pk
EUR 493.2
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 10L

91-200-79 1/pk
EUR 446.4
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 50L

91-200-81 1/pk
EUR 570
Description: Flexible Containers; Bioprocess Bags and Parts

Mouse ES Cell Culture Media

M16101 500 mL
EUR 1336.8

6-well Cell Culture Plates

abx090688-50plates 50 plates
EUR 571.2

6-well Cell Culture Plates

abx090688-6plates 6 plates
EUR 142.8

6-Well Cell Culture Plates

25-105 100 Plates/Unit
EUR 119.75
Description: Flat Bottom Wells, TC Treated

HEPES. cell culture tested - kg

PM-B2093/1000 kg
EUR 146.69

6-well Cell Culture Plates

abx090688-100l 100 µl
EUR 125

6-well Cell Culture Plates

abx090688-1ml 1 ml Ask for price

6-well Cell Culture Plates

abx090688-200l 200 µl
EUR 306.25

12-Well Cell Culture Plates

25-106 100 Plates/Unit
EUR 123.27
Description: Flat Bottom Wells, TC Treated

24-Well Cell Culture Plates

25-107 100 Plates/Unit
EUR 125.99
Description: Flat Bottom Wells, TC Treated

48-Well Cell Culture Plates

25-108 100 Plates/Unit
EUR 138.07
Description: Flat Bottom Wells, TC Treated

96-Well Cell Culture Plates

25-109 100 plates
EUR 117.72
Description: Flat Bottom Wells, TC Treated

96-Well Cell Culture Plates

25-221 100 plates
EUR 139.75
Description: U-Bottom Wells, TC Treated

Cell Culture Inserts, 6-Well

25-281 6 Inserts/Plate, 24/Unit
EUR 71.41
Description: 1µm PC Membrane, Sterilized

Cell Culture Inserts, 6-Well

25-282 6 Inserts/Plate, 24/Unit
EUR 71.41
Description: 3µm PC Membrane, Sterilized

Cell Culture Inserts, 6-Well

25-283 6 Inserts/Plate, 24/Unit
EUR 71.41
Description: 8µm PC Membrane, Sterilized

Imiquimod for cell culture, 98%

75054 250 Mg
EUR 133.22
Description: Part B1

Fish Serum (cell culture grade)

GWB-AE89C9 10 ml Ask for price

HEPES. cell culture tested - 100g

PM-B2093/100 100g
EUR 23.21

HEPES. cell culture tested - 250g

PM-B2093/250 250g
EUR 44

HEPES. cell culture tested - 500g

PM-B2093/500 500g
EUR 80.08

CHO Cell Culture Growth Media

MR1015 1 bottle
EUR 199.95
Description: CHO Cell Culture Growth Media (450mL)

Cell Culture Inserts, 12-Well

25-285 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 3µm PC Membrane, Sterilized

Cell Culture Inserts, 12-Well

25-286 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 8µm PC Membrane, Sterilized

Cell Culture Inserts, 24-Well

25-287 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 1µm PC Membrane, Sterilized

Cell Culture Inserts, 24-Well

25-288 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 3µm PC Membrane, Sterilized

Cell Culture Inserts, 24-Well

25-289 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 8µm PC Membrane, Sterilized

Yeast Extract for cell culture

52184 100 Gms
EUR 9.41
Description: Part D

Yeast Extract for cell culture

52184-1 500 Gms
EUR 24.8
Description: Part D

Nunc Cell Culture Plate Holder

LBI10241 each
EUR 227.81

ITS Cell Culture Supplement(100×)

ITSS-10201 10mL
EUR 59

Human CD34+ Cell Culture Medium

P771 8 rxn
EUR 299

EZ-LINE Cell culture Dishes Tissue culture treated, 60X15mm, 500 per case

SP31171 1CS, 500UNIT
EUR 230.89

EZ-LINE Cell culture Dishes Tissue culture treated, 90x20mm, 200 per case

SP31212 1CS, 200UNIT
EUR 162.61

Cell Culture Freezing Medium (FBS)

cAP-22 50ml
EUR 108.9

100 mm Cell Culture Dish Holder

LBI10235 each
EUR 227.81

35 mm Cell Culture Dish Holder

LBI10233 each
EUR 227.81

60 mm Cell Culture Dish Holder

LBI10234 each
EUR 227.81

3D Cell Culture Matrix BME Kit

K518-100 each
EUR 744

UltraPure Water, Cell Culture Tested

W9200-045 450ml
EUR 96

Cell Culture Supernatant Diluent Kit

DKC100 96 Tests
EUR 50

CardioDISC (Scaffold for cell culture)

C-01-01-EX 12 sheets Ask for price
Description: The CardioDISC (Scaffold for cell culture) is available in Europe and for worldwide shipping via Gentaur.

DMSO Bio-Max, Cell Culture Grade

40470005-1 50 mL
EUR 94.72

DMSO Bio-Max, Cell Culture Grade

40470005-2 100 mL
EUR 158.19

DMSO Bio-Max, Cell Culture Grade

40470005-3 25 mL
EUR 53.53

DMSO Bio-Max, Cell Culture Grade

40470005-4 250 mL
EUR 271.78

500mL Water Cell Culture Grade - PK6

25-055-CV PK6
EUR 66.15

Gibco CHO Cell Culture Medium - EACH

220253 EACH
EUR 64.48

Dimethylsulfoxide (DMSO) for Cell Culture

MBS6120680-100mL 100mL
EUR 395

Dimethylsulfoxide (DMSO) for Cell Culture

MBS6120680-25mL 25mL
EUR 210

Dimethylsulfoxide (DMSO) for Cell Culture

MBS6120680-50mL 50mL
EUR 290

Dimethylsulfoxide (DMSO) for Cell Culture

MBS6120680-5x10mL 5x10mL
EUR 260

Dimethylsulfoxide (DMSO) for Cell Culture

MBS6120680-5x5mL 5x5mL
EUR 210

Ultrafiltration Water, cell culture level

PB180518-500mL 500 mL
EUR 15
Description: Supplements & Reagents

Ultrafiltration Water, cell culture level

PB180518 500mL
EUR 15
Description: Others

Ultrafiltration Water, cell culture level

MBS2567648-500mL 500mL
EUR 85

Ultrafiltration Water, cell culture level

MBS2567648-5x500mL 5x500mL
EUR 370

Cell Culture Water Pyrogen free - 100ml

LM-T1707/100 100ml
EUR 2.59

Cell Culture Water Pyrogen free - 1000ml

LM-T1707/1000 1000ml
EUR 5.45

Cell Culture Water Pyrogen free - 500ml

LM-T1707/500 500ml
EUR 3.19

Pluronic F-127 *Cell culture tested *

MBS8584854-100mg 100mg
EUR 115

Pluronic F-127 *Cell culture tested *

MBS8584854-1g 1g
EUR 165

Pluronic F-127 *Cell culture tested *

MBS8584854-5x1g 5x1g
EUR 680

Cell Culture Freezing Medium (NON-FBS)

cAP-22B 50ml
EUR 128.7

1L Water Cell Culture Grade WFI - PK6

25-055-CM PK6
EUR 95.85

3D Cell Culture Gel, hard (Col-Tgel)

P720H-10 10 mL
EUR 389

3D Cell Culture Gel, hard (Col-Tgel)

P720H-2 2 mL
EUR 189

3D Cell Culture Gel, soft (Col-Tgel)

P720S-10 10 mL
EUR 389

3D Cell Culture Gel, soft (Col-Tgel)

P720S-2 2 mL
EUR 189

Gelatin Solution , 0.1%, Cell culture tested

CA087-100 1L
EUR 116.4

Mouse Epithelial Cell culture Medium Kit

ABM-TM4285 500 ml Basal medium with the growth factor supplement Ask for price
Description: Epithelial Cell Medium is a complete medium designed for the culture of epithelial cells. It was tested and optimized with epithelial cell growth and proliferation in vitro. It is formulated for use with 5% CO2 and 95% air in a humidified incubator.The medium consists of 500 ml of basal medium (containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals), supplemented with epithelial cell growth supplement, antibiotics, and fetal bovine serum.Epithelial Cell Medium Supplement Kit includes:- 0.5 ML RI-Transferrin-Selenium (ITS)- 0.5 ML EGF- 5.0 ML L-Glutamine- 5.0 ML Antibiotic-Antimycotic Solution- 10.0 ML FBS*Before use, add Supplement Kit into 500ml basal medium.

Lipoproteins, Low Density, HP,Cell Culture

12-16-120412-TC-1mg 1mg
EUR 85

Lipoproteins, Low Density, HP,Cell Culture

12-16-120412-TC-5mg 5mg
EUR 350

3D Cell Culture Gel, medium (Col-Tgel)

P720M-10 10 mL
EUR 389

3D Cell Culture Gel, medium (Col-Tgel)

P720M-2 2 mL
EUR 189

Ampicillin sodium salt, cell culture grade

GA0283 5g
EUR 173.88

Ampicillin sodium salt, cell culture grade

GA0283-100 100
EUR 189.8

Ampicillin sodium salt, cell culture grade

GA0283-100G 100 g
EUR 265.2

Ampicillin sodium salt, cell culture grade

GA0283-25 25
EUR 71.1

Ampicillin sodium salt, cell culture grade

GA0283-25G 25 g
EUR 122.4

Ampicillin sodium salt, cell culture grade

GA0283-5 5
EUR 23.8

Ampicillin sodium salt, cell culture grade

GA0283-5G 5 g
EUR 64.8

Sodium Bicarbonate. cell culture tested - kg

PM-S2011/1 kg
EUR 10.4

Lipoproteins, High Density, HP, Cell Culture

12-16-080412-TC-1mg 1mg
EUR 80

Lipoproteins, High Density, HP, Cell Culture

12-16-080412-TC-5mg 5mg
EUR 340

Sodium Bicarbonate. cell culture tested - 500g

PM-S2011/500 500g
EUR 6.71

BD Biocoat 10cm cell culture dish - PK10

354469 PK10
EUR 126.9

Ceraplex, Premium Cell Culture Suplement(10X)

C3318-010 100ml Ask for price

Ceraplex, Premium Cell Culture Suplement(10X)

C3318-050 500ml Ask for price

Human B Cell Culture and Expansion Kit

BC1001-1 1 package
EUR 5200

3D Cell Culture Matrix Duo-Matrix Kit

K519-100 each
EUR 718.8

3D Cell Culture Non-Enzymatic Cell Harvesting Kit

K982-100 each
EUR 326.4

GeneMATRIX CELL CULTURE DNA Purification Kit

E3555-01 50preps
EUR 95.92
Description: Kit for isolation of DNA from human and animal cell cultures

GeneMATRIX CELL CULTURE DNA Purification Kit

E3555-02 150preps
EUR 270.32
Description: Kit for isolation of DNA from human and animal cell cultures

Carcinoembryonic Antigen (CEA) 95% Cell Culture

GWB-7FE58C 0.1 mg Ask for price

Carcinoembryonic Antigen (CEA) 95% Cell Culture

GWB-B045F9 1 mg Ask for price

Albumin, Recombinant Human - Cell Culture Grade

MBS172027-INQUIRE INQUIRE Ask for price

Pluronic® F-127 *Cell culture tested *

20050-10g 10 g
EUR 86
Description: Pluronic® F-127 is a nonionic surfactant that is 100% active and relatively non-toxic to cells, and frequently used with dye AM esters to improve their water solubility.

40X9mm Cell Culture Plastic Dishes 20/PK

DS001-40 1PK, 20UNIT
EUR 64.18

60X12mm Cell Culture Plastic Dishes 20/PK

DS001-60 1PK, 20UNIT
EUR 64.7

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