The European Collection of Authenticated Cell Cultures (ECACC) was established in 1984 as a cell
culture collection to service the research community and provide an International Depository Authority
recognised patent depository for Europe. Over the last three decades ECACC has expanded and diversified to become one of the premier collections of authenticated cell cultures in the world and this remains the core of ECACC’s business. The collections currently hold over 40,000 cell lines representing 45 different species, 50 tissue types, 300 HLA types, 450 monoclonal antibodies and at least 800 genetic disorders.
The development and maintenance of such a diverse collection has inevitably produced a high level of
specialist knowledge and this has enabled ECACC to position itself as a centre of expertise in all aspects
of cell culture. ECACC has developed a comprehensive range of cell culture services and diversified into
new product areas such as extracted from cell lines.

ECACC is the largest of four collections of clinically significant organisms, maintained by Public Health
England (PHE). The other collections are the National Collection of Type Cultures (NCTC), the National
Collection of Pathogenic Viruses (NCPV), and the National Collection of Pathogenic Fungi (NCPF).

 

Design and Equipment for the Cell Culture Laboratory 2.1 Laboratory Design

Perhaps one of the most undervalued aspects of tissue culture is the need
to design the facility to ensure that good quality material is produced in a
safe and efficient manner. Most tissue culture is undertaken in laboratories
that have been adapted for the purpose and in conditions that are not ideal.
However, as long as a few basic guidelines are adopted this should not
compromise the work.

Cell Culture
Cell Culture

Traditional methods of central nervous system culture

Cell and tissue culture techniques have been central to neuroscience research and the advancing knowledge of key mechanisms underpinning central nervous system (CNS) development, function and malfunction. These methods have permitted investigation of cellular level functions that would be infeasible to carry out routinely in vivo. Not only are animal studies often more time consuming and expensive, the complexity of the in vivo environment can make it difficult to isolate and control confounding variables in order to investigate specific cell and molecular features. In some cases inaccessibility of relevant tissue renders such studies completely impossible.

One of the earliest reports of in vitro CNS cell culture was carried out by Harrison, who observed neurite extension in fragments of frog medullary tube cultured using the hanging drop method, which involves the inversion of tissue-containing solution that has been placed on a cover slip to create a hanging drop [1]. The study was pivotal in representing one of the first examples of CNS tissue being grown ex vivo. By the mid-20th century, human CNS tissue culture had become more common and was often performed in either hanging drop or roller tube formats [2,3]. Studies carried out using roller tube methods involved growing neural tissue explants on rotating surfaces to allow alternating exposure to culture medium and gaseous phases.

In 1955, Cavanaugh became the first to report the application of monolayer culture using neurons derived from chick embryos and then, two decades later by the mid-1970s, monolayer CNS tissue culture had begun to gain popularity. The monolayer cell culture technique relied on dissociation of brain tissues using trypsin or collagenase to form single cell suspensions which were then grown on various glass or plastic substrates. These studies were used to identify morphological, molecular and electrophysiological features of neurons and other CNS relevant cell types in vitro (reviewed in).

Conventional monolayer culture methods have also been referred to as two-dimensional (2D) because they require cell adherence to a flat surface, to which culture medium is applied allowing access to nutrients that enable cell survival and proliferation. While healthy cells remain attached to the surface, necrotic cells become detached and are easily removed during media change. Monolayer tissue culture has since been widely adopted as a routine method for assaying cell activity. Although glass was initially the predominant culture substrate of choice, clear polystyrene has taken precedence due to its cost efficiency, ease of visual analysis, and ability to promote cell adherence without the use of coatings..

 

ExoStep Cell Culture

ExoS-25-C9 25 test
EUR 660.6

Supreme Plant Tissue Culture Grade Agar

PCTA100 500g
EUR 144
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

Supreme Plant Tissue Culture Grade Agar

PCTA500 1000g
EUR 180
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

Hepes Cell Culture Grade

AK3268-0250 250g Ask for price

Hepes Cell Culture Grade

AK3268-0500 500g Ask for price

Hepes Cell Culture Grade

AK3268-1000 1kg Ask for price

Ethanolamine, Cell Culture tested

CA071-010 100ml
EUR 116.4

Water, Cell Culture Grade

CA018-600 6x1000ml
EUR 148.8

Human MEP Cell Culture Medium (human megakaryocyte–erythroid progenitor cell culture medium)

P770 - Ask for price

ROCKER CELL CULTURE BAG, 20L

91-200-78 1/pk
EUR 493.2
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 10L

91-200-79 1/pk
EUR 446.4
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 2L

91-200-80 1/pk
EUR 450
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 50L

91-200-81 1/pk
EUR 570
Description: Flexible Containers; Bioprocess Bags and Parts

6-well Cell Culture Plates

abx090688-50plates 50 plates
EUR 571.2

6-well Cell Culture Plates

abx090688-6plates 6 plates
EUR 142.8

CardioDISC (Scaffold for cell culture)

C-01-01-EX 12 sheets Ask for price
Description: The CardioDISC (Scaffold for cell culture) is available in Europe and for worldwide shipping via Gentaur.

Mouse ES Cell Culture Media

M16101 500 mL
EUR 1336.8

Human CD34+ Cell Culture Medium

P771 - Ask for price

UltraPure Water, Cell Culture Tested

W9200-045 450ml
EUR 96

Gibco CHO Cell Culture Medium

220253 EACH
EUR 50.05

500mL Water Cell Culture Grade

25-055-CV PK6
EUR 52.67

Greiner culture flasks tissue culture treated

C6231-200EA PK200
EUR 189.24

Gelatin Solution , 0.1%, Cell culture tested

CA087-100 1L
EUR 116.4

Ceraplex, Premium Cell Culture Suplement(10X)

C3318-010 100ml Ask for price

Ceraplex, Premium Cell Culture Suplement(10X)

C3318-050 500ml Ask for price

Ampicillin sodium salt, cell culture grade

GA0283-100G 100 g
EUR 265.2

Ampicillin sodium salt, cell culture grade

GA0283-25G 25 g
EUR 122.4

Ampicillin sodium salt, cell culture grade

GA0283-5G 5 g
EUR 64.8

3D Cell Culture Matrix BME Kit

K518-100
EUR 744

Tranfferrin, Human Recombinant, Cell culture tested

T4600-100 100mg
EUR 303.6

Tranfferrin, Human Recombinant, Cell culture tested

T4600-101 1g
EUR 1226.4

Tranfferrin, Human Recombinant, Cell culture tested

T4600-110 10g
EUR 9456

Multifuge X4R Pro cell culture package

75016066 EACH
EUR 19671.84

Multifuge X4 Pro cell culture package

75016067 EACH
EUR 14603.4

BD Biocoat 10cm cell culture dish

354469 PK10
EUR 101.46

1L Water Cell Culture Grade WFI

25-055-CM PK6
EUR 80.94

EZ-LINE Cell culture Dishes Tissue culture treated, 60X15mm, 500 per case

SP31171 1CS, 500UNIT
EUR 230.89

EZ-LINE Cell culture Dishes Tissue culture treated, 90x20mm, 200 per case

SP31212 1CS, 200UNIT
EUR 162.61

3D Cell Culture Non-Enzymatic Cell Harvesting Kit

K982-100
EUR 326.4

ExoStepTM Culture + Standard

ExoS-25-CST9 25 test
EUR 871.2

culture flask 250ml

FLA4286 PK10
EUR 251.21

culture flask 500ml

FLA4288 PK5
EUR 123.67

culture flask 1000ml

FLA4290 PK2
EUR 60.93

culture flask 2000ml

FLA4292 EACH
EUR 40.58

CORNING® ENDOTHELIAL CELL CULTURE MEDIUM, KIT

355054 1/pk
EUR 478.8
Description: Advanced Cells - DL; Adv Cell Misc Custom - DL

Pluronic® F-127 *Cell culture tested *

20050 10 g
EUR 138

Exosome Purification and Detection Kit (Cell Culture)

abx290025-25tests 25 tests
EUR 777.6

40X9mm Cell Culture Plastic Dishes 20/PK

DS001-40 1PK, 20UNIT
EUR 64.18

60X12mm Cell Culture Plastic Dishes 20/PK

DS001-60 1PK, 20UNIT
EUR 64.7

90X15mm Cell Culture Plastic Dishes 10/PK

DS001-90 1PK, 10UNIT
EUR 65.02

Exosome Isolation kit (for cell culture media)

P100 - Ask for price

Exosome Isolation kit (for cell culture media)

P100L - Ask for price

Water, GlenCell™, suitable for cell culture

GK3940-1L 1 l
EUR 79.2

L-Alanine, GlenCell, suitable for cell culture

GM0361-1KG 1 kg
EUR 303.6

L-Arginine, GlenCell, suitable for cell culture

GM1092-100G 100 g
EUR 69.6

L-Arginine, GlenCell, suitable for cell culture

GM1092-1KG 1 kg
EUR 204

L-Arginine, GlenCell, suitable for cell culture

GM1092-250G 250 g
EUR 93.6

L-Arginine, GlenCell, suitable for cell culture

GM1092-500G 500 g
EUR 132

HEPES, 99.5%, GlenCell, suitable for cell culture

GM5581-1KG 1 kg
EUR 370.8

L-Phenylalanine, GlenCell, suitable for cell culture

GM7895-1KG 1 kg
EUR 342

L-Glutamine, GlenCell, suitable for cell culture

GM8887-1KG 1 kg
EUR 294

L-Glutamine, GlenCell, suitable for cell culture

GM8887-500G 500 g
EUR 170.4

ExoFACS? Kit for Cell Culture Media Exosomes

K1236-20
EUR 973.2

3D Cell Culture Matrix Alginate Hydrogel Kit

K517-100
EUR 666

3D Cell Culture Matrix Duo-Matrix Kit

K519-100
EUR 718.8

3D Cell Culture Gel, hard (Col-Tgel)

P720H-10 - Ask for price

3D Cell Culture Gel, hard (Col-Tgel)

P720H-2 - Ask for price

3D Cell Culture Gel, medium (Col-Tgel)

P720M-10 - Ask for price

3D Cell Culture Gel, medium (Col-Tgel)

P720M-2 - Ask for price

3D Cell Culture Gel, soft (Col-Tgel)

P720S-10 - Ask for price

3D Cell Culture Gel, soft (Col-Tgel)

P720S-2 - Ask for price

SL4R Plus Centrifuge Cell Culture TX1000 Package

75016075 EACH
EUR 17439.72

Exosome Isolation kit (for cell culture media)

P100S - Ask for price

Falcon 6-well Cell Culture Plates tissue-culture treated polystyrene flat-bottom with lid

353224 PK36
EUR 116.28

3D Cell Culture HTS Cell Viability Complete Assay Kit

K948-100
EUR 411.6

Apotransferrin tissue culture grade

30-AA15TC 100 mg
EUR 152.4
Description: Apotransferrin, Human Plasma, Tissue Culture Grade

PetakaG3 FLAT Culture Devices

Petaka-FLAT 300 Petaka Devices
EUR 12196.8

PetakaG3 HOT Culture Devices

Petaka-HOT 300 Petaka Devices
EUR 12196.8

PetakaG3 LOT Culture Devices

Petaka-LOT 300 Petaka Devices
EUR 12196.8

Suspension Culture Flask 25cm

169900 PK200
EUR 305.52

Culture Tube 75x10mm Borosilicate

TES5120 PK1000
EUR 54.83

Culture Tube 75x12mm Borosilicate

TES5122 PK1000
EUR 58.14

Culture Tube 100x13mm Borosilicate

TES5124 PK1000
EUR 86.64

Culture Tube 100x16mm Borosilicate

TES5128 PK1000
EUR 112.86

Culture Tube 150x16mm Borosilicate

TES5132 PK1000
EUR 163.02

Culture Tube 150x18mm Borosilicate

TES5135 PK500
EUR 123.12

Culture Tube 150x20mm Borosilicate

TES5136 PK500
EUR 291.84

Techne 125ml Culture Vessel

TIS4090 EACH
EUR 427.5

Techne 250ml Culture Vessel

TIS4100 EACH
EUR 427.5

Techne 1L Culture Vessel

TIS4120 EACH
EUR 682.86

TPP Culture dish 40x11mm

TIS7100 PK900
EUR 513

TPP Culture dish 96x21mm

TIS7104 PK240
EUR 188.1

TPP Culture dish 146x21mm

TIS7106 PK100
EUR 216.6

Probenecid *Cell culture tested* *CAS 57-66-9*

20060 10x72 mg
EUR 138

Insect Cell Medium: TNM-FH Insect Culture Medium

ABP-MED-10001 1 liter Ask for price

Insect Cell Medium: Serum-Free Insect Culture Medium

ABP-MED-10002 1 liter Ask for price

Dimethyl Sulfoxide, Cell culture tested, >99.7% , 0.2um filtered

D2680-001 10ml
EUR 100.8

Dimethyl Sulfoxide, Cell culture tested, >99.7% , 0.2um filtered

D2680-002 25ml
EUR 133.2

Dimethyl Sulfoxide, Cell culture tested, >99.7% , 0.2um filtered

D2680-010 100ml
EUR 246

Pluronic F-68, 10% Sol, Cell Culture tested

CA072-010 100ml
EUR 105.6

Exosome Isolation kit (for stem cell culture media)

P107 - Ask for price

Exosome Isolation kit (for stem cell culture media)

P107S - Ask for price

N-Acetyl-L-cysteine (>99%, cell culture grade)

NALC15-1 1 g
EUR 270

N-Acetyl-L-cysteine (>99%, cell culture grade)

NALC15-5 5 g
EUR 489.6

Hydrochloric acid 36%, GlenCell, suitable for cell culture

GK5744-100ML 100 ml
EUR 74.4

Hydrochloric acid 36%, GlenCell, suitable for cell culture

GK5744-500ML 500 ml
EUR 151.2

L-Leucine, GlenCell™, suitable for cell culture

GM0001-1KG 1 kg
EUR 250.8

L-Tryptophan, GlenCell™, suitable for cell culture

GM0192-1KG 1 kg
EUR 591.6

L-Arginine hydrochloride, GlenCell, suitable for cell culture

GM1372-1KG 1 kg
EUR 189.6

L-Valine, GlenCell™, suitable for cell culture

GM3894-1KG 1 kg
EUR 313.2

L-Threonine, GlenCell™, suitable for cell culture

GM4012-1KG 1 kg
EUR 294

L-Serine, GlenCell™, suitable for cell culture

GM7000-1KG 1 kg
EUR 572.4

L-Isoleucine, GlenCell™, suitable for cell culture

GM8967-1KG 1 kg
EUR 534

L-Proline, GlenCell™, suitable for cell culture

GM9897-1KG 1 kg
EUR 361.2

Insulin, Human Recombinant, Cell culture tested, from E.coli

I4500-025 25mg
EUR 522

Insulin, Human Recombinant, Cell culture tested, from E.coli

I4500-101 1g
EUR 5406

Insulin, Human Recombinant, Cell culture tested, from E.coli

I4500-250 250mg
EUR 1226.4

Insulin, Human Recombinant, Cell culture tested, from Yeast

I4600-001 1g
EUR 562.8

Insulin, Human Recombinant, Cell culture tested, from Yeast

I4600-005 5g
EUR 2247.6

Insulin, Human Recombinant, Cell culture tested, from Yeast

I4600-010 10g
EUR 4110

VEX Exosome Isolation Reagent (from cell culture media)

R601 50 ml
EUR 706.8

Inactivated VZV Antigen (Human Embryo Lung Cell Culture)

VAng-0650Lsx-inquire inquire Ask for price
Description: VZV (Strain Ellen), inactivated by Beta-propiolactone. 7.61 mg/mL.

Recombinant CA 15-3 Protein (Cell Culture Supernatant)

VAng-Wyb8629-inquire inquire Ask for price
Description: CA 15-3 (Breast Cancer Antigen), Calibrator Grade, recombinant protein from Cell Culture Supernatant, 10003 Units/mL.

EZ-LINE Cell culture Dishes Tissue culture treated, 35X10mm, PS, Sterile TC treated, 500 per case

SP31146 1CS, 500UNIT
EUR 208.27

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135 96 assays
EUR 985.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 4027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 1027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 4179.6
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

293AAV Cell Line

AAV-100 1 vial
EUR 609.6
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293AD Cell Line

AD-100 1 vial
EUR 553.2
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

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