The European Collection of Authenticated Cell Cultures (ECACC) was established in 1984 as a cell
culture collection to service the research community and provide an International Depository Authority
recognised patent depository for Europe. Over the last three decades ECACC has expanded and diversified to become one of the premier collections of authenticated cell cultures in the world and this remains the core of ECACC’s business. The collections currently hold over 40,000 cell lines representing 45 different species, 50 tissue types, 300 HLA types, 450 monoclonal antibodies and at least 800 genetic disorders.
The development and maintenance of such a diverse collection has inevitably produced a high level of
specialist knowledge and this has enabled ECACC to position itself as a centre of expertise in all aspects
of cell culture. ECACC has developed a comprehensive range of cell culture services and diversified into
new product areas such as extracted from cell lines.

ECACC is the largest of four collections of clinically significant organisms, maintained by Public Health
England (PHE). The other collections are the National Collection of Type Cultures (NCTC), the National
Collection of Pathogenic Viruses (NCPV), and the National Collection of Pathogenic Fungi (NCPF).

 

Design and Equipment for the Cell Culture Laboratory 2.1 Laboratory Design

Perhaps one of the most undervalued aspects of tissue culture is the need
to design the facility to ensure that good quality material is produced in a
safe and efficient manner. Most tissue culture is undertaken in laboratories
that have been adapted for the purpose and in conditions that are not ideal.
However, as long as a few basic guidelines are adopted this should not
compromise the work.

Cell Culture
Cell Culture

Traditional methods of central nervous system culture

Cell and tissue culture techniques have been central to neuroscience research and the advancing knowledge of key mechanisms underpinning central nervous system (CNS) development, function and malfunction. These methods have permitted investigation of cellular level functions that would be infeasible to carry out routinely in vivo. Not only are animal studies often more time consuming and expensive, the complexity of the in vivo environment can make it difficult to isolate and control confounding variables in order to investigate specific cell and molecular features. In some cases inaccessibility of relevant tissue renders such studies completely impossible.

One of the earliest reports of in vitro CNS cell culture was carried out by Harrison, who observed neurite extension in fragments of frog medullary tube cultured using the hanging drop method, which involves the inversion of tissue-containing solution that has been placed on a cover slip to create a hanging drop [1]. The study was pivotal in representing one of the first examples of CNS tissue being grown ex vivo. By the mid-20th century, human CNS tissue culture had become more common and was often performed in either hanging drop or roller tube formats [2,3]. Studies carried out using roller tube methods involved growing neural tissue explants on rotating surfaces to allow alternating exposure to culture medium and gaseous phases.

In 1955, Cavanaugh became the first to report the application of monolayer culture using neurons derived from chick embryos and then, two decades later by the mid-1970s, monolayer CNS tissue culture had begun to gain popularity. The monolayer cell culture technique relied on dissociation of brain tissues using trypsin or collagenase to form single cell suspensions which were then grown on various glass or plastic substrates. These studies were used to identify morphological, molecular and electrophysiological features of neurons and other CNS relevant cell types in vitro (reviewed in).

Conventional monolayer culture methods have also been referred to as two-dimensional (2D) because they require cell adherence to a flat surface, to which culture medium is applied allowing access to nutrients that enable cell survival and proliferation. While healthy cells remain attached to the surface, necrotic cells become detached and are easily removed during media change. Monolayer tissue culture has since been widely adopted as a routine method for assaying cell activity. Although glass was initially the predominant culture substrate of choice, clear polystyrene has taken precedence due to its cost efficiency, ease of visual analysis, and ability to promote cell adherence without the use of coatings..

 

ExoStep Cell Culture

ExoS-25-C9 25 test
EUR 550.5

Supreme Plant Tissue Culture Grade Agar

PCTA100 500g
EUR 120
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

Supreme Plant Tissue Culture Grade Agar

PCTA500 1000g
EUR 150
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

Water, Cell Culture Grade

CA018-600 6x1000ml
EUR 124

Ethanolamine, Cell Culture tested

CA071-010 100ml
EUR 97

Hepes Cell Culture Grade

AK3268-0250 250g Ask for price

Hepes Cell Culture Grade

AK3268-0500 500g Ask for price

Hepes Cell Culture Grade

AK3268-1000 1kg Ask for price

Human MEP Cell Culture Medium (human megakaryocyte–erythroid progenitor cell culture medium)

P770 - Ask for price

Mouse ES Cell Culture Media

M16101 500 mL
EUR 1114

CardioDISC (Scaffold for cell culture)

C-01-01-EX 12 sheets Ask for price
Description: The CardioDISC (Scaffold for cell culture) is available in Europe and for worldwide shipping via Gentaur.

Human CD34+ Cell Culture Medium

P771 - Ask for price

ROCKER CELL CULTURE BAG, 20L

91-200-78 1/pk
EUR 411
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 10L

91-200-79 1/pk
EUR 372
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 2L

91-200-80 1/pk
EUR 375
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 50L

91-200-81 1/pk
EUR 475
Description: Flexible Containers; Bioprocess Bags and Parts

6-well Cell Culture Plates

abx090688-50plates 50 plates
EUR 476

6-well Cell Culture Plates

abx090688-6plates 6 plates
EUR 119

UltraPure Water, Cell Culture Tested

W9200-045 450ml
EUR 80

Ampicillin sodium salt, cell culture grade

GA0283-100G 100 g
EUR 221

Ampicillin sodium salt, cell culture grade

GA0283-25G 25 g
EUR 102

Ampicillin sodium salt, cell culture grade

GA0283-5G 5 g
EUR 54

Ceraplex, Premium Cell Culture Suplement(10X)

C3318-010 100ml Ask for price

Ceraplex, Premium Cell Culture Suplement(10X)

C3318-050 500ml Ask for price

Gelatin Solution , 0.1%, Cell culture tested

CA087-100 1L
EUR 97

Tranfferrin, Human Recombinant, Cell culture tested

T4600-100 100mg
EUR 253

Tranfferrin, Human Recombinant, Cell culture tested

T4600-101 1g
EUR 1022

Tranfferrin, Human Recombinant, Cell culture tested

T4600-110 10g
EUR 7880

3D Cell Culture Matrix BME Kit

K518-100
EUR 620

EZ-LINE Cell culture Dishes Tissue culture treated, 60X15mm, 500 per case

SP31171 1CS, 500UNIT
EUR 192.41

EZ-LINE Cell culture Dishes Tissue culture treated, 90x20mm, 200 per case

SP31212 1CS, 200UNIT
EUR 135.51

3D Cell Culture Non-Enzymatic Cell Harvesting Kit

K982-100
EUR 272

Pluronic® F-127 *Cell culture tested *

20050 10 g
EUR 115

40X9mm Cell Culture Plastic Dishes 20/PK

DS001-40 1PK, 20UNIT
EUR 53.48

60X12mm Cell Culture Plastic Dishes 20/PK

DS001-60 1PK, 20UNIT
EUR 53.92

90X15mm Cell Culture Plastic Dishes 10/PK

DS001-90 1PK, 10UNIT
EUR 54.18

Water, GlenCell™, suitable for cell culture

GK3940-1L 1 l
EUR 66

HEPES, 99.5%, GlenCell, suitable for cell culture

GM5581-1KG 1 kg
EUR 309

L-Alanine, GlenCell, suitable for cell culture

GM0361-1KG 1 kg
EUR 253

L-Arginine, GlenCell, suitable for cell culture

GM1092-100G 100 g
EUR 58

L-Arginine, GlenCell, suitable for cell culture

GM1092-1KG 1 kg
EUR 170

L-Arginine, GlenCell, suitable for cell culture

GM1092-250G 250 g
EUR 78

L-Arginine, GlenCell, suitable for cell culture

GM1092-500G 500 g
EUR 110

L-Phenylalanine, GlenCell, suitable for cell culture

GM7895-1KG 1 kg
EUR 285

L-Glutamine, GlenCell, suitable for cell culture

GM8887-1KG 1 kg
EUR 245

L-Glutamine, GlenCell, suitable for cell culture

GM8887-500G 500 g
EUR 142

Exosome Isolation kit (for cell culture media)

P100 - Ask for price

Exosome Isolation kit (for cell culture media)

P100L - Ask for price

Exosome Isolation kit (for cell culture media)

P100S - Ask for price

3D Cell Culture Gel, hard (Col-Tgel)

P720H-10 - Ask for price

3D Cell Culture Gel, hard (Col-Tgel)

P720H-2 - Ask for price

3D Cell Culture Gel, medium (Col-Tgel)

P720M-10 - Ask for price

3D Cell Culture Gel, medium (Col-Tgel)

P720M-2 - Ask for price

3D Cell Culture Gel, soft (Col-Tgel)

P720S-10 - Ask for price

3D Cell Culture Gel, soft (Col-Tgel)

P720S-2 - Ask for price

CORNING® ENDOTHELIAL CELL CULTURE MEDIUM, KIT

355054 1/pk
EUR 399
Description: Advanced Cells - DL; Adv Cell Misc Custom - DL

Exosome Purification and Detection Kit (Cell Culture)

abx290025-25tests 25 tests
EUR 648

ExoFACS? Kit for Cell Culture Media Exosomes

K1236-20
EUR 811

3D Cell Culture Matrix Alginate Hydrogel Kit

K517-100
EUR 555

3D Cell Culture Matrix Duo-Matrix Kit

K519-100
EUR 599

ExoStepTM Culture + Standard

ExoS-25-CST9 25 test
EUR 726

3D Cell Culture HTS Cell Viability Complete Assay Kit

K948-100
EUR 343

Recombinant CA 15-3 Protein (Cell Culture Supernatant)

VAng-Wyb8629-inquire inquire Ask for price
Description: CA 15-3 (Breast Cancer Antigen), Calibrator Grade, recombinant protein from Cell Culture Supernatant, 10003 Units/mL.

Hydrochloric acid 36%, GlenCell, suitable for cell culture

GK5744-100ML 100 ml
EUR 62

Hydrochloric acid 36%, GlenCell, suitable for cell culture

GK5744-500ML 500 ml
EUR 126

L-Serine, GlenCell™, suitable for cell culture

GM7000-1KG 1 kg
EUR 477

L-Leucine, GlenCell™, suitable for cell culture

GM0001-1KG 1 kg
EUR 209

L-Tryptophan, GlenCell™, suitable for cell culture

GM0192-1KG 1 kg
EUR 493

L-Arginine hydrochloride, GlenCell, suitable for cell culture

GM1372-1KG 1 kg
EUR 158

L-Valine, GlenCell™, suitable for cell culture

GM3894-1KG 1 kg
EUR 261

L-Threonine, GlenCell™, suitable for cell culture

GM4012-1KG 1 kg
EUR 245

Insulin, Human Recombinant, Cell culture tested, from E.coli

I4500-025 25mg
EUR 435

Insulin, Human Recombinant, Cell culture tested, from E.coli

I4500-101 1g
EUR 4505

Insulin, Human Recombinant, Cell culture tested, from E.coli

I4500-250 250mg
EUR 1022

Insulin, Human Recombinant, Cell culture tested, from Yeast

I4600-001 1g
EUR 469

Insulin, Human Recombinant, Cell culture tested, from Yeast

I4600-005 5g
EUR 1873

Insulin, Human Recombinant, Cell culture tested, from Yeast

I4600-010 10g
EUR 3425

L-Isoleucine, GlenCell™, suitable for cell culture

GM8967-1KG 1 kg
EUR 445

L-Proline, GlenCell™, suitable for cell culture

GM9897-1KG 1 kg
EUR 301

Pluronic F-68, 10% Sol, Cell Culture tested

CA072-010 100ml
EUR 88

Dimethyl Sulfoxide, Cell culture tested, >99.7% , 0.2um filtered

D2680-001 10ml
EUR 84

Dimethyl Sulfoxide, Cell culture tested, >99.7% , 0.2um filtered

D2680-002 25ml
EUR 111

Dimethyl Sulfoxide, Cell culture tested, >99.7% , 0.2um filtered

D2680-010 100ml
EUR 205

Exosome Isolation kit (for stem cell culture media)

P107 - Ask for price

Exosome Isolation kit (for stem cell culture media)

P107S - Ask for price

Inactivated VZV Antigen (Human Embryo Lung Cell Culture)

VAng-0650Lsx-inquire inquire Ask for price
Description: VZV (Strain Ellen), inactivated by Beta-propiolactone. 7.61 mg/mL.

N-Acetyl-L-cysteine (>99%, cell culture grade)

NALC15-1 1 g
EUR 225

N-Acetyl-L-cysteine (>99%, cell culture grade)

NALC15-5 5 g
EUR 408

Insect Cell Medium: TNM-FH Insect Culture Medium

ABP-MED-10001 1 liter Ask for price

Insect Cell Medium: Serum-Free Insect Culture Medium

ABP-MED-10002 1 liter Ask for price

3D Culture HTS Cell Viability Assay Kit (Colorimetric)

K2032-100 100 assays
EUR 379

VEX Exosome Isolation Reagent (from cell culture media)

R601 50 ml
EUR 589

PetakaG3 FLAT Culture Devices

Petaka-FLAT 300 Petaka Devices
EUR 10164

PetakaG3 HOT Culture Devices

Petaka-HOT 300 Petaka Devices
EUR 10164

PetakaG3 LOT Culture Devices

Petaka-LOT 300 Petaka Devices
EUR 10164

Apotransferrin tissue culture grade

30-AA15TC 100 mg
EUR 127
Description: Apotransferrin, Human Plasma, Tissue Culture Grade

EZ-LINE Cell culture Dishes Tissue culture treated, 35X10mm, PS, Sterile TC treated, 500 per case

SP31146 1CS, 500UNIT
EUR 173.56

CORNING® T-CELL CULTURE SUPPLEMENT WITH CONA (IL-2 CULTURE SUPPLEMENT), RAT, 100ML, 1/PACK

354115 1/pk
EUR 582
Description: Cell Culture Reagents; Growth Supplements - DL

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135 96 assays
EUR 821
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 3356
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 856
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 3483
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

L-Aspartic acid, GlenCell™, suitable for cell culture

GM0348-1KG 1 kg
EUR 110

L-Histidine monohydrochloride monohydrate, GlenCell, suitable for cell culture

GM2021-1KG 1 kg
EUR 285

L-Glutamic acid, GlenCell™, suitable for cell culture

GM8976-1KG 1 kg
EUR 102

L-Lysine monohydrochloride, GlenCell™, suitable for cell culture

GM9011-1KG 1 kg
EUR 110

Bovine Serum Albumin, GlenCell™, suitable for cell culture

GX7143-1KG 1 kg
EUR 1457

Bovine Serum Albumin, GlenCell™, suitable for cell culture

GX7143-500G 500 g
EUR 796

3D Cell Culture Gel 2ml x(soft+medium+hard)

P720SMH-6 - Ask for price

Bovine Collagen Type I (Skin, >97%) for cell culture

CLGN16-N-1 5 mg
EUR 529

3D Cell Culture Ready-to-Use Scaffold Complete Kit

K990-100
EUR 615

Lipoproteins, High Density, Human Plasma, Suitable for Cell Culture

7853-1000
EUR 224

Lipoproteins, Low Density, Human Plasma, Suitable for Cell Culture

7854-1000
EUR 218

293RTV Cell Line

RV-100 1 vial
EUR 508
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 508
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 508
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293AD Cell Line

AD-100 1 vial
EUR 461
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 856
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325-5 5 x 96 assays
EUR 3361
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size

CBA-135-T 24 assays
EUR 432
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

293/GFP Cell Line

AKR-200 1 vial
EUR 572
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 572
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 572
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 572
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 572
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 572

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 572

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 572

Tissue Culture Plate, 12 Well

TCP20-12 1 UNIT
EUR 53.48

Tissue Culture Plate, 48 Well

TCP20-48 1 UNIT
EUR 53.48

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