The European Collection of Authenticated Cell Cultures (ECACC) was established in 1984 as a cell
culture collection to service the research community and provide an International Depository Authority
recognised patent depository for Europe. Over the last three decades ECACC has expanded and diversified to become one of the premier collections of authenticated cell cultures in the world and this remains the core of ECACC’s business. The collections currently hold over 40,000 cell lines representing 45 different species, 50 tissue types, 300 HLA types, 450 monoclonal antibodies and at least 800 genetic disorders.
The development and maintenance of such a diverse collection has inevitably produced a high level of
specialist knowledge and this has enabled ECACC to position itself as a centre of expertise in all aspects
of cell culture. ECACC has developed a comprehensive range of cell culture services and diversified into
new product areas such as extracted from cell lines.

ECACC is the largest of four collections of clinically significant organisms, maintained by Public Health
England (PHE). The other collections are the National Collection of Type Cultures (NCTC), the National
Collection of Pathogenic Viruses (NCPV), and the National Collection of Pathogenic Fungi (NCPF).

 

Design and Equipment for the Cell Culture Laboratory 2.1 Laboratory Design

Perhaps one of the most undervalued aspects of tissue culture is the need
to design the facility to ensure that good quality material is produced in a
safe and efficient manner. Most tissue culture is undertaken in laboratories
that have been adapted for the purpose and in conditions that are not ideal.
However, as long as a few basic guidelines are adopted this should not
compromise the work.

Cell Culture
Cell Culture

Traditional methods of central nervous system culture

Cell and tissue culture techniques have been central to neuroscience research and the advancing knowledge of key mechanisms underpinning central nervous system (CNS) development, function and malfunction. These methods have permitted investigation of cellular level functions that would be infeasible to carry out routinely in vivo. Not only are animal studies often more time consuming and expensive, the complexity of the in vivo environment can make it difficult to isolate and control confounding variables in order to investigate specific cell and molecular features. In some cases inaccessibility of relevant tissue renders such studies completely impossible.

One of the earliest reports of in vitro CNS cell culture was carried out by Harrison, who observed neurite extension in fragments of frog medullary tube cultured using the hanging drop method, which involves the inversion of tissue-containing solution that has been placed on a cover slip to create a hanging drop [1]. The study was pivotal in representing one of the first examples of CNS tissue being grown ex vivo. By the mid-20th century, human CNS tissue culture had become more common and was often performed in either hanging drop or roller tube formats [2,3]. Studies carried out using roller tube methods involved growing neural tissue explants on rotating surfaces to allow alternating exposure to culture medium and gaseous phases.

In 1955, Cavanaugh became the first to report the application of monolayer culture using neurons derived from chick embryos and then, two decades later by the mid-1970s, monolayer CNS tissue culture had begun to gain popularity. The monolayer cell culture technique relied on dissociation of brain tissues using trypsin or collagenase to form single cell suspensions which were then grown on various glass or plastic substrates. These studies were used to identify morphological, molecular and electrophysiological features of neurons and other CNS relevant cell types in vitro (reviewed in).

Conventional monolayer culture methods have also been referred to as two-dimensional (2D) because they require cell adherence to a flat surface, to which culture medium is applied allowing access to nutrients that enable cell survival and proliferation. While healthy cells remain attached to the surface, necrotic cells become detached and are easily removed during media change. Monolayer tissue culture has since been widely adopted as a routine method for assaying cell activity. Although glass was initially the predominant culture substrate of choice, clear polystyrene has taken precedence due to its cost efficiency, ease of visual analysis, and ability to promote cell adherence without the use of coatings..

 

ExoStep Cell Culture

ExoS-25-C9 25 test
EUR 660.6

Hepes Cell Culture Grade

AK3268-0250 250g Ask for price

Hepes Cell Culture Grade

AK3268-0500 500g Ask for price

Hepes Cell Culture Grade

AK3268-1000 1kg Ask for price

Water, Cell Culture Grade

CA018-600 6x1000ml
EUR 148.8

CELL CULTURE GRADE WATER

IB42010 1L
EUR 41.13
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42011 6x1L
EUR 239.38
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42012 12x1L
EUR 469.02
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42020 2L
EUR 59.67
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42021 6x2L
EUR 342.87
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42030 10L
EUR 194.72
Description: JUG W/HANDL 0.1 MICRON STERILE

CRFK CELL TUBE CULTURE

63-0600 each Ask for price

ROCKER CELL CULTURE BAG, 2L

91-200-80 1/pk
EUR 450
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 20L

91-200-78 1/pk
EUR 493.2
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 10L

91-200-79 1/pk
EUR 446.4
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 50L

91-200-81 1/pk
EUR 570
Description: Flexible Containers; Bioprocess Bags and Parts

Mouse ES Cell Culture Media

M16101 500 mL
EUR 1336.8

Ethanolamine, Cell Culture tested

CA071-010 100ml
EUR 116.4

6-well Cell Culture Plates

abx090688-50plates 50 plates
EUR 571.2

6-well Cell Culture Plates

abx090688-6plates 6 plates
EUR 142.8

Gibco CHO Cell Culture Medium

220253 EACH
EUR 57.32

500mL Water Cell Culture Grade

25-055-CV PK6
EUR 58.8

Human CD34+ Cell Culture Medium

P771 - Ask for price

ITS Cell Culture Supplement(100×)

ITSS-10201 10mL
EUR 59

Human MEP Cell Culture Medium (human megakaryocyte–erythroid progenitor cell culture medium)

P770 - Ask for price

EZ-LINE Cell culture Dishes Tissue culture treated, 60X15mm, 500 per case

SP31171 1CS, 500UNIT
EUR 230.89

EZ-LINE Cell culture Dishes Tissue culture treated, 90x20mm, 200 per case

SP31212 1CS, 200UNIT
EUR 162.61

3D Cell Culture Matrix BME Kit

K518-100 each
EUR 744

1L Water Cell Culture Grade WFI

25-055-CM PK6
EUR 85.2

UltraPure Water, Cell Culture Tested

W9200-045 450ml
EUR 96

CardioDISC (Scaffold for cell culture)

C-01-01-EX 12 sheets Ask for price
Description: The CardioDISC (Scaffold for cell culture) is available in Europe and for worldwide shipping via Gentaur.

BD Biocoat 10cm cell culture dish

354469 PK10
EUR 112.8

DMSO Bio-Max, Cell Culture Grade

40470005-1 50 mL
EUR 94.72

DMSO Bio-Max, Cell Culture Grade

40470005-2 100 mL
EUR 158.19

DMSO Bio-Max, Cell Culture Grade

40470005-3 25 mL
EUR 53.53

DMSO Bio-Max, Cell Culture Grade

40470005-4 250 mL
EUR 271.78

Ultrafiltration Water, cell culture level

PB180518-500mL 500 mL
EUR 15
Description: Supplements & Reagents

Multifuge X4 Pro cell culture package

75016067 EACH
EUR 16141.2

3D Cell Culture Gel, hard (Col-Tgel)

P720H-10 - Ask for price

3D Cell Culture Gel, hard (Col-Tgel)

P720H-2 - Ask for price

3D Cell Culture Gel, soft (Col-Tgel)

P720S-10 - Ask for price

3D Cell Culture Gel, soft (Col-Tgel)

P720S-2 - Ask for price

Multifuge X4R Pro cell culture package

75016066 EACH
EUR 21742.8

Gelatin Solution , 0.1%, Cell culture tested

CA087-100 1L
EUR 116.4

3D Cell Culture Gel, medium (Col-Tgel)

P720M-10 - Ask for price

3D Cell Culture Gel, medium (Col-Tgel)

P720M-2 - Ask for price

Ampicillin sodium salt, cell culture grade

GA0283-100G 100 g
EUR 265.2

Ampicillin sodium salt, cell culture grade

GA0283-25G 25 g
EUR 122.4

Ampicillin sodium salt, cell culture grade

GA0283-5G 5 g
EUR 64.8

Ampicillin sodium salt, cell culture grade

GA0283-100 100
EUR 189.8

Ampicillin sodium salt, cell culture grade

GA0283-25 25
EUR 71.1

Ampicillin sodium salt, cell culture grade

GA0283-5 5
EUR 23.8

Pluronic® F-127 *Cell culture tested *

20050 10 g
EUR 86

3D Cell Culture Matrix Duo-Matrix Kit

K519-100 each
EUR 718.8

Human B Cell Culture and Expansion Kit

BC1001-1 1 package
EUR 6500

40X9mm Cell Culture Plastic Dishes 20/PK

DS001-40 1PK, 20UNIT
EUR 64.18

Pluronic® F-127 *Cell culture tested *

20050-10g 10 g
EUR 86
Description: Pluronic® F-127 is a nonionic surfactant that is 100% active and relatively non-toxic to cells, and frequently used with dye AM esters to improve their water solubility.

Ceraplex, Premium Cell Culture Suplement(10X)

C3318-010 100ml Ask for price

Ceraplex, Premium Cell Culture Suplement(10X)

C3318-050 500ml Ask for price

60X12mm Cell Culture Plastic Dishes 20/PK

DS001-60 1PK, 20UNIT
EUR 64.7

90X15mm Cell Culture Plastic Dishes 10/PK

DS001-90 1PK, 10UNIT
EUR 65.02

3D Cell Culture Non-Enzymatic Cell Harvesting Kit

K982-100 each
EUR 326.4

EZ-LINE Cell culture Dishes Tissue culture treated, 35X10mm, PS, Sterile TC treated, 500 per case

SP31146 1CS, 500UNIT
EUR 208.27

Water, GlenCell™, suitable for cell culture

GK3940-1L 1 l
EUR 79.2

ExoFACS? Kit for Cell Culture Media Exosomes

K1236-20 each
EUR 973.2

HEPES, 99.5%, GlenCell, suitable for cell culture

GM5581-1KG 1 kg
EUR 370.8

Water, GlenCell™, suitable for cell culture

GK3940-1 1
EUR 55.4

HS27 CELLS TUBE CULTURE

87-0600 each Ask for price
Description: Single or Mixed ; FreshFrozen

Pluronic F-68, 10% Sol, Cell Culture tested

CA072-010 100ml
EUR 105.6

3D Cell Culture Matrix Alginate Hydrogel Kit

K517-100 each
EUR 666

Exosome Isolation kit (for cell culture media)

P100 - Ask for price

Exosome Isolation kit (for cell culture media)

P100L - Ask for price

Exosome Isolation kit (for cell culture media)

P100S - Ask for price

Probenecid *Cell culture tested* *CAS 57-66-9*

20060 10x72 mg
EUR 86

Probenecid *Cell culture tested* *CAS 57-66-9*

20060-10x72mg 10x72 mg
EUR 86
Description: Probenecid is an inhibitor of organic-anion transporters located in cell membranes.

Greiner culture flasks tissue culture treated

C6231-200EA PK200
EUR 231.6

3D Cell Culture HTS Cell Viability Complete Assay Kit

K948-100 each
EUR 411.6

Tranfferrin, Human Recombinant, Cell culture tested

T4600-100 100mg
EUR 303.6

Tranfferrin, Human Recombinant, Cell culture tested

T4600-101 1g
EUR 1226.4

Tranfferrin, Human Recombinant, Cell culture tested

T4600-110 10g
EUR 9456

CORNING® ENDOTHELIAL CELL CULTURE MEDIUM, KIT

355054 1/pk
EUR 478.8
Description: Advanced Cells - DL; Adv Cell Misc Custom - DL

L-Alanine, GlenCell, suitable for cell culture

GM0361-1KG 1 kg
EUR 303.6

3D Cell Culture Gel 2ml x(soft+medium+hard)

P720SMH-6 - Ask for price

L-Arginine, GlenCell, suitable for cell culture

GM1092-100G 100 g
EUR 69.6

L-Arginine, GlenCell, suitable for cell culture

GM1092-1KG 1 kg
EUR 204

L-Arginine, GlenCell, suitable for cell culture

GM1092-250G 250 g
EUR 93.6

L-Arginine, GlenCell, suitable for cell culture

GM1092-500G 500 g
EUR 132

SL4R Plus Centrifuge Cell Culture TX1000 Package

75016075 EACH
EUR 18357.6

L-Glutamine, GlenCell, suitable for cell culture

GM8887-1KG 1 kg
EUR 294

L-Glutamine, GlenCell, suitable for cell culture

GM8887-500G 500 g
EUR 170.4

HEPES, 99.5%, GlenCell™, suitable for cell culture

GM5581-1 1
EUR 276.9

N-Acetyl-L-cysteine (>99%, cell culture grade)

NALC15-1 1 g
EUR 270

N-Acetyl-L-cysteine (>99%, cell culture grade)

NALC15-5 5 g
EUR 489.6

L-Valine, GlenCell™, suitable for cell culture

GM3894-1KG 1 kg
EUR 313.2

L-Serine, GlenCell™, suitable for cell culture

GM7000-1KG 1 kg
EUR 572.4

L-Valine, GlenCell™, suitable for cell culture

GM3894-1 1
EUR 229.3

L-Serine, GlenCell™, suitable for cell culture

GM7000-1 1
EUR 442.9

L-Serine, GlenCell™, suitable for cell culture

GM7000-100 100
EUR 88.6

Culture flask CELLine CL 350

DD150102B PK5
EUR 2312.4

Exosome Purification and Detection Kit (Cell Culture)

abx290025-25tests 25 tests
EUR 777.6

Exosome Isolation kit (for stem cell culture media)

P107S - Ask for price

Exosome Isolation kit (for stem cell culture media)

P107 - Ask for price

L-Leucine, GlenCell™, suitable for cell culture

GM0001-1KG 1 kg
EUR 250.8

L-Proline, GlenCell™, suitable for cell culture

GM9897-1KG 1 kg
EUR 361.2

L-Leucine, GlenCell™, suitable for cell culture

GM0001-1 1
EUR 177.9

L-Alanine, GlenCell™, suitable for cell culture

GM0361-1 1
EUR 221.5

L-Proline, GlenCell™, suitable for cell culture

GM9897-1 1
EUR 268.9

L-Proline, GlenCell™, suitable for cell culture

GM9897-100 100
EUR 63.3

Bovine Collagen Type I (Skin, >97%) for cell culture

CLGN16-N-1 5 mg
EUR 634.8

L-Phenylalanine, GlenCell, suitable for cell culture

GM7895-1KG 1 kg
EUR 342

L-Arginine, GlenCell™, suitable for cell culture

GM1092-1 1
EUR 138.5

L-Arginine, GlenCell™, suitable for cell culture

GM1092-100 100
EUR 27.8

L-Arginine, GlenCell™, suitable for cell culture

GM1092-250 250
EUR 47.4

L-Arginine, GlenCell™, suitable for cell culture

GM1092-500 500
EUR 79.1

L-Threonine, GlenCell™, suitable for cell culture

GM4012-1KG 1 kg
EUR 294

L-Threonine, GlenCell™, suitable for cell culture

GM4012-1 1
EUR 213.7

L-Glutamine, GlenCell™, suitable for cell culture

GM8887-1 1
EUR 213.7

L-Glutamine, GlenCell™, suitable for cell culture

GM8887-500 500
EUR 110.7

L-Tryptophan, GlenCell™, suitable for cell culture

GM0192-1KG 1 kg
EUR 591.6

L-Isoleucine, GlenCell™, suitable for cell culture

GM8967-1KG 1 kg
EUR 534

L-Tryptophan, GlenCell™, suitable for cell culture

GM0192-1 1
EUR 458.8

L-Tryptophan, GlenCell™, suitable for cell culture

GM0192-100 100
EUR 87.1

L-Tryptophan, GlenCell™, suitable for cell culture

GM0192-500 500
EUR 261.6

L-Isoleucine, GlenCell™, suitable for cell culture

GM8967-1 1
EUR 411.3

Recombinant CA 15-3 Protein (Cell Culture Supernatant)

VAng-Wyb8629-inquire inquire Ask for price
Description: CA 15-3 (Breast Cancer Antigen), Calibrator Grade, recombinant protein from Cell Culture Supernatant, 10003 Units/mL.

VEX Exosome Isolation Reagent (from cell culture media)

R601 50 ml
EUR 706.8

Dimethyl Sulfoxide, Cell culture tested, >99.7% , 0.2um filtered

D2680-001 10ml
EUR 100.8

Dimethyl Sulfoxide, Cell culture tested, >99.7% , 0.2um filtered

D2680-002 25ml
EUR 133.2

Dimethyl Sulfoxide, Cell culture tested, >99.7% , 0.2um filtered

D2680-010 100ml
EUR 246

Inactivated VZV Antigen (Human Embryo Lung Cell Culture)

VAng-0650Lsx-inquire inquire Ask for price
Description: VZV (Strain Ellen), inactivated by Beta-propiolactone. 7.61 mg/mL.

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