Assay principle
The coated well immunoenzymatic assay for the quantitative measurement of analyte utilizes a multiclonal anti‐analyte antibody and an analyte‐HRP conjugate. The assay sample and buffer are incubated together with analyte‐HRP conjugate in pre‐coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme‐substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader.
The intensity of the color is inversely proportional to the analyte concentration since analyte from samples and analyte ‐HRP conjugate competes for the anti-analyte antibody binding site. Since the number of sites is limited, as more sites are occupied by analyanalytete from the sample, fewer sites are left to bind analyte‐HRP conjugate. Standards of known analyte concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of the analyte. The analyte concentration in each sample is interpolated from this standard curve.
Manufactured and distributed by:
NovaTein Biosciences 310 West Cummings Park Woburn, MA 01801 US
Materials supplied
1
Microelisa Stripplate
96 well
6
Chromogenic Substrate A
6ml X 1vial
2
Standard
1.0 ml X 6 vials
7
Chromogenic Substrate B
6ml X 1 vial
3
100 X Wash Solution
10ml X 1vial
8
Stop Solution
6ml X 1 via
4
Lysis Buffer Solution
6ml X 1vial
9
Specification
1
5
5 HRP‐Conjugate Reagent
6ml X 1 vial
Note: Standard (S1 → S6) concentration was followed by:Variable with different kit
microelisa stripplate
Sample collection and storage
Serum‐Use a serum separator tube (SST) and allow samples to clot for 30 minutes before a centrifugation for 15minutes at approximately 1000 x g. Remove serum and perform the assay immediately or aliquot and store samples at ‐20 °C or ‐80 °C.
Plasma‐Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 x g at 2‐8 °C within 30minutes of collection. Store samples at ‐20°C or ‐80°C.Avoid repeated freeze‐thaw cycles.
Cell culture fluid and other biological fluids‐Remove particulates by centrifugation and assay immediately or aliquot and store samples at ‐20°C or ‐80°C. Avoid repeated freeze‐thaw cycles.
NOTE: The Lysis Buffer Solution is used only when the sample is cell culture fluid & body fluid & tissue homogenate; if the sample is serum or blood plasma, then the Lysis Buffer Solution is a superfluous reagent. Serum, plasma, and cell culture fluid samples to be used within 7 days may be stored at 2‐8 °C, otherwise samples must be stored at ‐20°C(≤2months) or ‐80°C(≤6months) to avoid loss of bioactivity and contamination. Avoid freeze‐thaw cycles. When performing the assay, warm up samples to room temperature slowly. DO NOT USE HEAT‐TREATED SAMPLES.
Materialsrequired but notsupplied
1.37 °C incubator
2.Standard plate reader capable of measuring absorbance at 450 nm.
3.Precision pipettes and disposable pipette tips
4.Distilled water
5.Multi‐channel pipettes, manifold dispenser or automated microplate washer.
6.Absorbent paper
Sample Preparation
1.Novateinbio is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient amount ofsamplesin advance.
2.Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
3.If the samples are not indicated in the manual, a preliminary experiment to determine the validity ofthe kitis necessary.
4 Owing to the possibility of mismatching between antigen from other resource and antibody used in our kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteinsfrom other manufacturers may not be recognized by our products.
5.Influenced by the factorsincluding cell viability, cell number and also sampling time,samplesfrom cell culture supernatant may not be detected by the kit
6.Fresh samples without long time storage is recommended for the test. Otherwise, protein degradation and denaturalization may occurin those samples and finally lead to wrong results.
Sterile filtered chicken serum, 0.2 micron, charcoal stripped
SC31-0050
Equitech
50 ml
EUR 196.56
Sterile filtered chicken serum, 0.2 micron,charcoal stripped
SC31-0100
Equitech
100 ml
EUR 223.08
Sterile filtered chicken serum, 0.2 micron, charcoal stripped
SC31-0500
Equitech
500 ml
EUR 293.28
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBM31-0100
Equitech
100 ml
EUR 308.88
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBM31-0500
Equitech
500 ml
EUR 780
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBU31-0100
Equitech
100 ml
EUR 354.12
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered
SFBU31-0500
Equitech
500 ml
EUR 900.12
Sterile filtered chicken serum, 0.2 micron, charcoal stripped, heat-inactivated
SC3132-0050
Equitech
50 ml
EUR 199.68
Sterile filtered chicken serum, 0.2 micron, charcoal stripped, heat-inactivated
SC3132-0100
Equitech
100 ml
EUR 226.2
Sterile filtered chicken serum, 0.2 micron, charcoal stripped, heat-inactivated
SC3132-0500
Equitech
500 ml
EUR 302.64
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBM3132-0100
Equitech
100 ml
EUR 330.72
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBM3132-0500
Equitech
500 ml
EUR 787.8
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBU3132-0100
Equitech
100 ml
EUR 360.36
Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate
SFBU3132-0500
Equitech
500 ml
EUR 907.92
CLC / Ipomoea Microelements (100X)
TS1044-1VL
EWC Diagnostics
1 unit
EUR 7.91
Description: CLC / Ipomoea Microelements (100X)
CLC / Ipomoea Microelements (100X)
TS1044-5VL
EWC Diagnostics
1 unit
EUR 36.01
Description: CLC / Ipomoea Microelements (100X)
DKW / Juglans Microelements (100X)
TS1049-1VL
EWC Diagnostics
1 unit
EUR 7.91
Description: DKW / Juglans Microelements (100X)
DKW / Juglans Microelements (100X)
TS1049-5VL
EWC Diagnostics
1 unit
EUR 36.01
Description: DKW / Juglans Microelements (100X)
Eriksson (ER) Microelements (100X)
TS1051-1VL
EWC Diagnostics
1 unit
EUR 7.91
Description: Eriksson (ER) Microelements (100X)
Eriksson (ER) Microelements (100X)
TS1051-5VL
EWC Diagnostics
1 unit
EUR 36.1
Description: Eriksson (ER) Microelements (100X)
De Greef & Jacobs Microelements (100X)
TS1047-1VL
EWC Diagnostics
1 unit
EUR 7.91
Description: De Greef & Jacobs Microelements (100X)
De Greef & Jacobs Microelements (100X)
TS1047-5VL
EWC Diagnostics
1 unit
EUR 36.1
Description: De Greef & Jacobs Microelements (100X)
Chée & Pool (C2D) Vitis Microelements (100X)
TS1040-1VL
EWC Diagnostics
1 unit
EUR 7.91
Description: Chée & Pool (C2D) Vitis Microelements (100X)
Chée & Pool (C2D) Vitis Microelements (100X)
TS1040-5VL
EWC Diagnostics
1 unit
EUR 36.01
Description: Chée & Pool (C2D) Vitis Microelements (100X)
Knudson C Orchid Microelements (100X)
TS1059-1VL
EWC Diagnostics
1 unit
EUR 8
Description: Knudson C Orchid Microelements (100X)
Knudson C Orchid Microelements (100X)
TS1059-5VL
EWC Diagnostics
1 unit
EUR 35.95
Description: Knudson C Orchid Microelements (100X)
Gamborg B5 Microelements Solution (100X)
PL004-1X100ML
EWC Diagnostics
1 unit
EUR 47.62
Description: Gamborg B5 Microelements Solution (100X)
Gamborg B5 Microelements Solution (100X)
PL004-5X100ML
EWC Diagnostics
1 unit
EUR 202.72
Description: Gamborg B5 Microelements Solution (100X)
Lindemann Orchid Microelements (100X)
TS1061-1VL
EWC Diagnostics
1 unit
EUR 7.91
Description: Lindemann Orchid Microelements (100X)
Lindemann Orchid Microelements (100X)
TS1061-5VL
EWC Diagnostics
1 unit
EUR 35.95
Description: Lindemann Orchid Microelements (100X)
Gamborg B5 / Kao Michayluk Microelements (100X)
TS1053-1VL
EWC Diagnostics
1 unit
EUR 7.91
Description: Gamborg B5 / Kao Michayluk Microelements (100X)
Gamborg B5 / Kao Michayluk Microelements (100X)
TS1053-5VL
EWC Diagnostics
1 unit
EUR 36.01
Description: Gamborg B5 / Kao Michayluk Microelements (100X)
Kao & Michayluk Microelements Solution (100X)
PL008-1X100ML
EWC Diagnostics
1 unit
EUR 50.51
Description: Kao & Michayluk Microelements Solution (100X)
Kao & Michayluk Microelements Solution (100X)
PL008-5X100ML
EWC Diagnostics
1 unit
EUR 209.16
Description: Kao & Michayluk Microelements Solution (100X)
Orchid Maintenance Microelements (100X)
TS1081-1VL
EWC Diagnostics
1 unit
EUR 8
Description: Orchid Maintenance Microelements (100X)
Orchid Maintenance Microelements (100X)
TS1081-5VL
EWC Diagnostics
1 unit
EUR 35.95
Description: Orchid Maintenance Microelements (100X)
Schenk & Hildebrandt Microelements (100X)
TS1088-1VL
EWC Diagnostics
1 unit
EUR 8
Description: Schenk & Hildebrandt Microelements (100X)
Schenk & Hildebrandt Microelements (100X)
TS1088-5VL
EWC Diagnostics
1 unit
EUR 35.95
Description: Schenk & Hildebrandt Microelements (100X)
AFTSpin MicroElute Fast RNA Extraction Kit
RK30124
Abclonal
50RXN
EUR 38.15
Nitsch & Nitsch Microelements Solution (100X)
PL014-1X100ML
EWC Diagnostics
1 unit
EUR 55.63
Description: Nitsch & Nitsch Microelements Solution (100X)
Nitsch & Nitsch Microelements Solution (100X)
PL014-5X100ML
EWC Diagnostics
1 unit
EUR 271.19
Description: Nitsch & Nitsch Microelements Solution (100X)
Murashige & Skoog Microelements Soution (100X)
PL012-1X100ML
EWC Diagnostics
1 unit
EUR 52.08
Description: Murashige & Skoog Microelements Soution (100X)
Murashige & Skoog Microelements Soution (100X)
PL012-5X100ML
EWC Diagnostics
1 unit
EUR 233.5
Description: Murashige & Skoog Microelements Soution (100X)
Gresshoff & Doy (DBM2) Microelements Solution (100X)
PL006-1X100ML
EWC Diagnostics
1 unit
EUR 48.47
Description: Gresshoff & Doy (DBM2) Microelements Solution (100X)
Gresshoff & Doy (DBM2) Microelements Solution (100X)
PL006-5X100ML
EWC Diagnostics
1 unit
EUR 213.44
Description: Gresshoff & Doy (DBM2) Microelements Solution (100X)
Linsmaier & Skoog Microelements Solution (100X)
PL010-1X100ML
EWC Diagnostics
1 unit
EUR 47.55
Description: Linsmaier & Skoog Microelements Solution (100X)
Linsmaier & Skoog Microelements Solution (100X)
PL010-5X100ML
EWC Diagnostics
1 unit
EUR 212.4
Description: Linsmaier & Skoog Microelements Solution (100X)
Anderson Rhododendron Microelements (100X)
TS1038-1VL
EWC Diagnostics
1 unit
EUR 7.91
Description: Anderson Rhododendron Microelements (100X)
Anderson Rhododendron Microelements (100X)
TS1038-5VL
EWC Diagnostics
1 unit
EUR 36.01
Description: Anderson Rhododendron Microelements (100X)
Linsmaier & Skoog / Murashige & Skoog Microelements (100X)
TS1063-1VL
EWC Diagnostics
1 unit
EUR 7.91
Description: Linsmaier & Skoog / Murashige & Skoog Microelements (100X)
Linsmaier & Skoog / Murashige & Skoog Microelements (100X)
TS1063-5VL
EWC Diagnostics
1 unit
EUR 35.66
Description: Linsmaier & Skoog / Murashige & Skoog Microelements (100X)
MicroElute PCR Clean Up Kit (100prep) (blister packing for columns)
FAMPK_001-1B
Favorgen
1 kit
EUR 56
MicroElute PCR Clean Up Kit (50prep) (blister packing for columns)
FAMPK_001B
Favorgen
1 kit
EUR 28
Kao & Michayluk Medium w/ Macroelements & Microelements; w/ Mac
PT037-10X1L
EWC Diagnostics
1 unit
EUR 11.48
Description: Kao & Michayluk Medium w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements
Kao & Michayluk Medium w/ Macroelements & Microelements; w/ Mac
PT037-25L
EWC Diagnostics
1 unit
EUR 14.96
Description: Kao & Michayluk Medium w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements
Kao & Michayluk Medium w/ Macroelements & Microelements; w/ Mac
PT037-5L
EWC Diagnostics
1 unit
EUR 4.99
Description: Kao & Michayluk Medium w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements
Vacin & Went Medium w/ Macroelements & Microelements; w/ Macroe
PT041-10X1L
EWC Diagnostics
1 unit
EUR 11.59
Description: Vacin & Went Medium w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements
Vacin & Went Medium w/ Macroelements & Microelements; w/ Macroe
PT041-25L
EWC Diagnostics
1 unit
EUR 18.5
Description: Vacin & Went Medium w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements
Vacin & Went Medium w/ Macroelements & Microelements; w/ Macroe
PT041-5L
EWC Diagnostics
1 unit
EUR 5.04
Description: Vacin & Went Medium w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements
Murashige & Skoog (Van der Salm Modification) Microelements (10
TS1074-1VL
EWC Diagnostics
1 unit
EUR 8.15
Description: Murashige & Skoog (Van der Salm Modification) Microelements (100X)
Murashige & Skoog (Van der Salm Modification) Microelements (10
TS1074-5VL
EWC Diagnostics
1 unit
EUR 36.57
Description: Murashige & Skoog (Van der Salm Modification) Microelements (100X)
Lindemann Orchid Medium w/ Macroelements & Microelements; w/ Ma
PT039-10X1L
EWC Diagnostics
1 unit
EUR 7.52
Description: Lindemann Orchid Medium w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements
Lindemann Orchid Medium w/ Macroelements & Microelements; w/ Ma
PT039-25L
EWC Diagnostics
1 unit
EUR 13.66
Description: Lindemann Orchid Medium w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements
Lindemann Orchid Medium w/ Macroelements & Microelements; w/ Ma
PT039-5L
EWC Diagnostics
1 unit
EUR 3.28
Description: Lindemann Orchid Medium w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements
NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg
PT107-10X1L
EWC Diagnostics
1 unit
EUR 16.05
Description: NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg B5 vitamins &Sucrose;w/o Agar
NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg
PT107-25L
EWC Diagnostics
1 unit
EUR 23.93
Description: NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg B5 vitamins &Sucrose;w/o Agar
NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg
PT107-5L
EWC Diagnostics
1 unit
EUR 6.98
Description: NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg B5 vitamins &Sucrose;w/o Agar
Tissue Total RNA MicroElure Kit (50prep) (blister packing for columns)
FATRM_001B
Favorgen
1 kit
EUR 62
Tissue Total RNA MicroElutei Kit (100prep) (blister packing for columns)
FATRM_001-1B
Favorgen
1 kit
EUR 106.5
Heller Medium w/ Macroelements & Microelements; w/ Macroelement
PT036-10X1L
EWC Diagnostics
1 unit
EUR 20.02
Description: Heller Medium w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements
Heller Medium w/ Macroelements & Microelements; w/ Macroelement
PT036-25L
EWC Diagnostics
1 unit
EUR 32.02
Description: Heller Medium w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements
Heller Medium w/ Macroelements & Microelements; w/ Macroelement
PT036-5L
EWC Diagnostics
1 unit
EUR 8.71
Description: Heller Medium w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements; w/ Macroelements & Microelements
MicroElute GEL/PCR Purification Kit (100prep) (blister packing for columns)
FAEPK_001-1B
Favorgen
1 kit
EUR 64.96
MicroElute GEL/PCR Purification Kit (50prep) (blister packing for columns)
FAEPK_001B
Favorgen
1 kit
EUR 33
MicroElute GEL Purification Kit (100prep) (blister packing for columns)
FAMGK_001-1B
Favorgen
1 kit
EUR 56
MicroElute GEL Purification Kit (50prep) (blister packing for columns)
FAMGK_001B
Favorgen
1 kit
EUR 28
Soil/ Stool RNA Extraction MicroElute Kit (50prep) (blister packing for columns)
FAFRK_001B-MICRO
Favorgen
1 kit
EUR 160
Tissue Genomic DNA Extraction MicroElute Kit (50prep), with Proteinase K Powder
FATGM-001
Favorgen
50 preps
EUR 202.8
Tissue Genomic DNA Extraction MicroElute Kit (100prep), with Proteinase K Powder
FATGM-001-1
Favorgen
100 preps
EUR 276
Tissue Genomic DNA Extraction MicroElute Kit (300prep), with Proteinase K Powder
FATGM-001-2
Favorgen
300 preps
EUR 528
Streptavidin-Coated Strip Microwell Plate (Flat)
R-1102
EpiGentek
Ask for price
Ask for price
MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, WHITE, FLAT BOTTOM, HIGH BINDING, NO LID, NONSTERILE, BULK
93923
CORNING
25/pk
EUR 763.2
Description: Stripwells; Stripwells
OPTIONAL HEAD FOR ONE MICROPLATE OR 64X0.2ML TUBES OR 8X0.2ML TUBE STRIPS
480102
CORNING
1/pk
EUR 75.6
Description: Lab Equipment; Shakers & Mixers
Tissue Genomic DNA Extraction MicroElute Kit (100prep), with Proteinase K Powder, (blister packing for columns)
FATGM_001-1B
Favorgen
1 kit
EUR 129
Tissue Genomic DNA Extraction MicroElute Kit (50prep), with Proteinase K Powder , (blister packing for columns)
FATGM_001B
Favorgen
1 kit
EUR 64
MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, HIGH BINDING, OEM, NO LID, NONSTERILE, BULK
92592
CORNING
25/pk
EUR 612
Description: Stripwells; OEM - Strips
MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, MEDIUM BINDING, OEM, NO LID, NONSTERILE, BULK
92593
CORNING
25/pk
EUR 814.8
Description: Stripwells; OEM - Strips
MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, LIGHT BLUE, FLAT BOTTOM, MEDIUM BINDING, NO LID, NONSTERILE, BULK
9548
CORNING
25/pk
EUR 1100.4
Description: Stripwells; Stripwells
MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, HIGH BINDING, NO LID, RED HOT STAMP FOIL, NONSTERILE, BULK
9459
CORNING
25/pk
EUR 1100.4
Description: Stripwells; Stripwells
MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, HIGH BINDING, NO LID, GRAY HOT STAMP FOIL, NONSTERILE, BULK
9467
CORNING
25/pk
EUR 1100.4
Description: Stripwells; Stripwells