Assay principle

The coated well immunoenzymatic assay for the quantitative measurement of analyte utilizes a multiclonal anti‐analyte antibody and an analyte‐HRP conjugate. The assay sample and buffer are incubated together with analyte‐HRP conjugate in pre‐coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme‐substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader.

The intensity of the color is inversely proportional to the analyte concentration since analyte from samples and analyte ‐HRP conjugate competes for the anti-analyte antibody binding site. Since the number of sites is limited, as more sites are occupied by analyanalytete from the sample, fewer sites are left to bind analyte‐HRP conjugate. Standards of known analyte concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of the analyte. The analyte concentration in each sample is interpolated from this standard curve.

Manufactured and distributed by:

NovaTein Biosciences 310 West Cummings Park Woburn, MA 01801 US

Materials supplied

1 Microelisa Stripplate 96 well 6 Chromogenic Substrate A 6ml X 1vial
2 Standard 1.0 ml X 6  vials 7 Chromogenic  Substrate B 6ml X 1 vial
3 100 X Wash Solution 10ml X 1vial 8 Stop Solution 6ml X 1 via
4 Lysis Buffer Solution 6ml X 1vial 9 Specification 1
5 5 HRP‐Conjugate Reagent 6ml X 1 vial

Note: Standard (S1 → S6) concentration was followed by:Variable with different kit

microelisa stripplate
microelisa stripplate

Sample collection and storage

  • Serum‐Use a serum separator tube (SST) and allow samples to clot for 30 minutes before a centrifugation for 15minutes at approximately 1000 x g. Remove serum and perform the assay immediately or aliquot and store samples at ‐20 °C or ‐80 °C.
  • Plasma‐Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 x g at 2‐8 °C within 30minutes of collection. Store samples at  ‐20°C or ‐80°C.Avoid repeated freeze‐thaw cycles.
  • Cell culture fluid and other biological fluids‐Remove particulates by centrifugation and assay immediately or aliquot and store samples at  ‐20°C or  ‐80°C. Avoid repeated freeze‐thaw cycles.
  • NOTE: The Lysis Buffer Solution is used only when the sample is cell culture fluid & body fluid & tissue homogenate; if the sample is serum or blood plasma, then the Lysis Buffer Solution is a superfluous reagent. Serum, plasma, and cell culture fluid samples to be used within 7 days may be stored at 2‐8 °C, otherwise samples must be stored at  ‐20°C(≤2months) or ‐80°C(≤6months) to avoid loss of bioactivity and contamination. Avoid freeze‐thaw cycles. When performing the assay, warm up samples to room temperature slowly. DO NOT USE HEAT‐TREATED SAMPLES.

Materialsrequired but notsupplied

  • 1.37 °C incubator
  • 2.Standard plate reader capable of measuring absorbance at 450 nm.
  • 3.Precision pipettes and disposable pipette tips
  • 4.Distilled water
  • 5.Multi‐channel pipettes, manifold dispenser or automated microplate washer.
  • 6.Absorbent paper

Sample Preparation

  • 1.Novateinbio is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient amount ofsamplesin advance.
  • 2.Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
  • 3.If the samples are not indicated in the manual, a preliminary experiment to determine the validity ofthe kitis necessary.
  • 4 Owing to the possibility of mismatching between antigen from other resource and antibody used in our kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteinsfrom other manufacturers may not be recognized by our products.
  • 5.Influenced by the factorsincluding cell viability, cell number and also sampling time,samplesfrom cell culture supernatant may not be detected by the kit
  • 6.Fresh samples without long time storage is recommended for the test. Otherwise, protein degradation and denaturalization may occurin those samples and finally lead to wrong results.

Sterile filtered chicken serum, 0.2 micron, charcoal stripped

SC31-0050 50 ml
EUR 196.56

Sterile filtered chicken serum, 0.2 micron,charcoal stripped

SC31-0100 100 ml
EUR 223.08

Sterile filtered chicken serum, 0.2 micron, charcoal stripped

SC31-0500 500 ml
EUR 293.28

MicroElute PCR Clean Up Kit (50prep)

FAMPK-001 50 preps
EUR 151.2

MicroElute PCR Clean Up Kit (200prep)

FAMPK-001-1 200 preps
EUR 224.4

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered

SFBM31-0100 100 ml
EUR 308.88

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered

SFBM31-0500 500 ml
EUR 780

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered

SFBU31-0100 100 ml
EUR 354.12

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered

SFBU31-0500 500 ml
EUR 900.12

Sterile filtered chicken serum, 0.2 micron, charcoal stripped, heat-inactivated

SC3132-0050 50 ml
EUR 199.68

Sterile filtered chicken serum, 0.2 micron, charcoal stripped, heat-inactivated

SC3132-0100 100 ml
EUR 226.2

Sterile filtered chicken serum, 0.2 micron, charcoal stripped, heat-inactivated

SC3132-0500 500 ml
EUR 302.64

MicroElute GEL Purification Kit (50prep)

FAMGK-001 50 preps
EUR 151.2

MicroElute GEL Purification Kit (200prep)

FAMGK-001-1 200 preps
EUR 224.4

MicroElute GEL/PCR Purification Kit (50prep)

FAEPK-001 50 preps
EUR 157.2

MicroElute GEL/PCR Purification Kit (200prep)

FAEPK-001-1 200 preps
EUR 249.6

MicroElute GEL/PCR Purification Kit (300prep)

FAEPK-001-2 300 preps
EUR 274.8

Soil/ Stool RNA Extraction MicroElute Kit (100prep)

FAFRK-001-MICRO 100 preps
EUR 363.6

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate

SFBM3132-0100 100 ml
EUR 330.72

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate

SFBM3132-0500 500 ml
EUR 787.8

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate

SFBU3132-0100 100 ml
EUR 360.36

Sterile filtered fetal bovine serum, charcoal stripped, triple 0.1 micron filtered, heat-inactivate

SFBU3132-0500 500 ml
EUR 907.92

Block for Microtube Strips (2.0mL) 6 strips

BLO1288 EACH
EUR 298.8

Block, for Microtube Strips (2.0ml) 6 strips

BSW2MTS 1 each
EUR 224

MicroElute PCR Clean Up Kit (100prep) (blister packing for columns)

FAMPK_001-1B 1 kit
EUR 56

MicroElute PCR Clean Up Kit (50prep) (blister packing for columns)

FAMPK_001B 1 kit
EUR 28

Tissue Total RNA MicroElure Kit (50prep) (blister packing for columns)

FATRM_001B 1 kit
EUR 62

Tissue Total RNA MicroElutei Kit (100prep) (blister packing for columns)

FATRM_001-1B 1 kit
EUR 106.5

MicroElute GEL/PCR Purification Kit (100prep) (blister packing for columns)

FAEPK_001-1B 1 kit
EUR 64.96

MicroElute GEL/PCR Purification Kit (50prep) (blister packing for columns)

FAEPK_001B 1 kit
EUR 33

MicroElute GEL Purification Kit (100prep) (blister packing for columns)

FAMGK_001-1B 1 kit
EUR 56

MicroElute GEL Purification Kit (50prep) (blister packing for columns)

FAMGK_001B 1 kit
EUR 28

Soil/ Stool RNA Extraction MicroElute Kit (50prep) (blister packing for columns)

FAFRK_001B-MICRO 1 kit
EUR 160

Tissue Genomic DNA Extraction MicroElute Kit (50prep), with Proteinase K Powder

FATGM-001 50 preps
EUR 202.8

Tissue Genomic DNA Extraction MicroElute Kit (100prep), with Proteinase K Powder

FATGM-001-1 100 preps
EUR 276

Tissue Genomic DNA Extraction MicroElute Kit (300prep), with Proteinase K Powder

FATGM-001-2 300 preps
EUR 528

Strips of 8 microtubes natural domed cap

DD45405 PK120
EUR 266.4

Microplate 12x8-Well Strip High Binding

GR238002 Case of 50
EUR 439

Microplate 12x8-Well Strip Medium Binding

GR238003 Case of 50
EUR 275

Strip of 8 flat optical microtubes cap natural colour

DD038735B PK300
EUR 264

Strip of 8 microtubes 0.2 mL with domed cap assorted colours

DD45412 PK120
EUR 268.8

Strip of 8 microtubes low profile natural flat cap ABgene

DD16389 PK120
EUR 430.8

Streptavidin-Coated Strip Microwell Plate (Flat) 

R-1102
  • EUR 361.02
  • EUR 204.60
  • 5/pk
  • 5/pk

468667 Thermo Microplate 12x8-Well Strip Nunc Maxixorp F8

GR238001 Case of 60
EUR 616

OPTIONAL HEAD FOR ONE MICROPLATE OR 64X0.2ML TUBES OR 8X0.2ML TUBE STRIPS

480102 1/pk
EUR 75.6
Description: Lab Equipment; Shakers & Mixers

Tissue Genomic DNA Extraction MicroElute Kit (100prep), with Proteinase K Powder, (blister packing for columns)

FATGM_001-1B 1 kit
EUR 129

Tissue Genomic DNA Extraction MicroElute Kit (50prep), with Proteinase K Powder , (blister packing for columns)

FATGM_001B 1 kit
EUR 64

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, WHITE, FLAT BOTTOM, HIGH BINDING, NO LID, NONSTERILE, BULK

43923 25/pk
EUR 770.4
Description: Stripwells; Stripwells

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, HIGH BINDING, NO LID, NONSTERILE, BULK

42592 25/pk
EUR 619.2
Description: Stripwells; Stripwells

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, WHITE, FLAT BOTTOM, HIGH BINDING, NO LID, NONSTERILE, BULK

93923 25/pk
EUR 763.2
Description: Stripwells; Stripwells

ELISA Microwell Strip Reader with built-in dot matrix printer

MSR-10 1
EUR 5613.6

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, LIGHT BLUE, FLAT BOTTOM, MEDIUM BINDING, NO LID, NONSTERILE, BULK

9548 25/pk
EUR 1100.4
Description: Stripwells; Stripwells

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, HIGH BINDING, OEM, NO LID, NONSTERILE, BULK

92592 25/pk
EUR 612
Description: Stripwells; OEM - Strips

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, MEDIUM BINDING, OEM, NO LID, NONSTERILE, BULK

92593 25/pk
EUR 814.8
Description: Stripwells; OEM - Strips

TIPS MICRO-E,0.5-10UL,NS,RK,96/960

4834 96/pk
EUR 105.6
Description: Non Filter Tips; Costar Specialty Tips

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, LIGHT GREEN, FLAT BOTTOM, MEDIUM BINDING, NO LID, NONSTERILE, BULK

9546 25/pk
EUR 1100.4
Description: Stripwells; Stripwells

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, HIGH BINDING, NO LID, RED HOT STAMP FOIL, NONSTERILE, BULK

9459 25/pk
EUR 1100.4
Description: Stripwells; Stripwells

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, HIGH BINDING, NO LID, GRAY HOT STAMP FOIL, NONSTERILE, BULK

9467 25/pk
EUR 1100.4
Description: Stripwells; Stripwells

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, HIGH BINDING, NO LID, PINK HOT STAMP FOIL, NONSTERILE, BULK

9468 25/pk
EUR 1100.4
Description: Stripwells; Stripwells

Corning 96 Well Clear Polystyrene High Bind Stripwell Microplate NO Lid Ns

2592 PK100
EUR 681.6

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, WHITE, FLAT BOTTOM, DNA-BIND®, NO LID, NONSTERILE, INDIVIDUALLY PACKAGED

2497 1/pk
EUR 1045.2
Description: Stripwells; Stripwells

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, HIGH BINDING, NO LID, NONSTERILE, BULK, SPECIAL PACKAGE

72592 25/pk
EUR 762
Description: Stripwells; OEM - Strips

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, HIGH BINDING, NO LID, YELLOW HOT STAMP FOIL, NONSTERILE, BULK

94611 25/pk
EUR 1080
Description: Stripwells; Stripwells

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, HIGH BINDING, NO LID, DARK GREEN HOT STAMP FOIL, NONSTERILE, BULK

94621 25/pk
EUR 1080
Description: Stripwells; Stripwells

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, HIGH BINDING, NO LID, BLACK HOT STAMP FOIL, NONSTERILE, BULK

9464 25/pk
EUR 1100.4
Description: Stripwells; Stripwells

MICROPLATE, 96 WELL STRIPWELL™, 8 WELL STRIPS, POLYSTYRENE, CLEAR, FLAT BOTTOM, HIGH BINDING, NO LID, DARK BLUE HOT STAMP FOIL, NONSTERILE, BULK

9466 25/pk
EUR 1100.4
Description: Stripwells; Stripwells

CK BRAIN STRIPPED 50 EA*

43005-2 50 EA
EUR 769.66

DNA-BIND 1 x 8 Stripwell; 96 Well Polystyrene Microplate without Lid Nonsterile

2506 PK50
EUR 841.32

WB Stripping Buffer

AR0153 100mL (for 10 assays for an 5 × 8.5cm blot)
EUR 133.2

Stripping Buffer

GR103020 500 mL
EUR 89

Goat Anti-human IgM plate (8 x 12 microwell strips coated wih anti-human IgG antibody) (1 plate)

MI20103 1 plate Ask for price
Description: Goat Anti-human IgM plate (8 x 12 microwell strips coated wih anti-human IgG antibody) (1 plate)

Western Stripper Kit

20920000-1 20 Blot (s)
EUR 82.93

Rabbit BRAIN STRIPPED MT 25 EA

41305-2 25 EA
EUR 409.48

Bovine BRAIN STRIPPED 5 EA*

57105-1 5 EA
EUR 977.15

West Ez Stripping Buffer

S2100-006 60ml
EUR 97.2

Blot Stripping Buffer 4X, pH 6.8

20960004-1 250 mL
EUR 23.24

Blot Stripping Buffer 4X, pH 6.8

20960004-2 500 mL
EUR 53.99

Blot Stripping Buffer II 4X, pH 2.5

20960005-1 250 mL
EUR 25.45

Blot Stripping Buffer II 4X, pH 2.5

20960005-2 500 mL
EUR 42.24

WB Stripping Solution

05364-55 500ML
EUR 116.9

Rabbit Anti-human IgG plate (8 x 12 microwell strips coated wih anti-human IgG antibody) (1 plate)

MI20109 1 plate Ask for price
Description: Rabbit Anti-human IgG plate (8 x 12 microwell strips coated wih anti-human IgG antibody) (1 plate)

Rabbit BRAINS STRIPPED 25 EA

41205-2 25 EA
EUR 409.48

Human IgG-RF stripper/Adsorbent (1 ml, sufficient for stripping 50 samples of 100 ul human serum/plasma)

RFS-1 1 ml
EUR 123.6

Corning 96 Well Clear High Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile

2481 PK100
EUR 938.4

Corning 96 Well Black High Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile

2483 PK100
EUR 975.6

Corning 96 Well White High Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile

2485 PK100
EUR 1017.6

COOLBOX XT PCR96 WORKSTATION WITH COOLRACK XT M-PCR FOR 6 ROWS OF PCR STRIP-WELLS AND UP TO 12 MICROFUGE TUBES.

432032 1/pk
EUR 950.4
Description: BioCision; BioCision CoolBox

WB Stripping Solution Strong

05677-65 500ML
EUR 142.8

Tris-SDS Stripping Buffer, pH 6.8

20920002-1 500 mL
EUR 24.17

Tris-SDS Stripping Buffer, pH 6.8

20920002-2 1 L
EUR 36.14

Tris-SDS Stripping Buffer, pH 6.8

20920002-3 4 L
EUR 115.76

Corning 96 Well Black Medium Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile

2482 PK100
EUR 956.4

Corning 96 Well White Medium Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile

2484 PK100
EUR 997.2

Corning 96 Well (1 x 8 Stripwell) Clear Flat Bottom TC-Treated Microplates Wrapped with Lid Sterile

9102 PK50
EUR 874.8

WB / Antibody Stripping Buffer

20-abx090656
  • EUR 226.80
  • EUR 276.00
  • 100 ml
  • 200 ml

WB / Antibody Stripping Buffer

abx090671-200ml 200 ml
EUR 226.8

101Bio WB Stripping Solution

P5W3 - Ask for price

WB Stripping Solution Trial Set

05680-21 1SET
EUR 25.2

Corning 96 Well White Polystyrene High Bind Stripwell Microplate 25 per Bag without Lid Nonsterile

3923 PK100
EUR 952.8

Corning 96 Well Black Polystyrene High Bind Stripwell Microplate 25 per Bag without Lid Nonsterile

3924 PK100
EUR 858

Dura Mater Stripping Forcep Angled

INS6134 EACH
EUR 112.8

Corning 96 Well Clear Polystyrene Not Treated Stripwell Microplate 25 per Bag without Lid Nonsterile

2593 PK100
EUR 681.6

Corning 96 Well White Polystyrene Medium Bind Stripwell Microplate 25 per Bag without Lid Nonsterile

3913 PK100
EUR 858

Corning 96 Well Black Polystyrene Medium Bind Stripwell Microplate 25 per Bag without Lid Nonsterile

3914 PK100
EUR 858

Cortisol ELISA Kit (1 Strip plate)

K003-H1 1x96 well plate
EUR 300

Cortisol ELISA Kit (5 Strip Plate)

K003-H5 5x96 well plate
EUR 1162

Oxytocin ELISA Kit (1 Strip Plate)

K048-H1 1x96 well plate
EUR 495

Oxytocin ELISA Kit (5 Strip Plate)

K048-H5 5x96 well plate
EUR 1905

2'3'-Cyclic GAMP ELISA Kit, (1 Strip Plate)

K067-H1 1x96 well plate
EUR 572

2'3'-Cyclic GAMP ELISA Kit, (5 Strip Plate)

K067-H5 5x96 well plate
EUR 2342

Dura Mater Stripping Forcep Straight

INS6132 EACH
EUR 112.8

Fetal Bovien Serum, Charcol Stripped

F0700-010 100ml
EUR 481.2

Fetal Bovien Serum, Charcol Stripped

F0700-050 500ml
EUR 1064.4

T-Pro Western Blot Stripping Reagent

JB11-K002 500ml/BT
EUR 213.6

West Ez Stripping Buffer, Economic Size

S2100-050 500ml
EUR 254.4

West Ez Stripping Buffer, Economic Size

S2100-100 2x500ml
EUR 336

Corning 96 Well Clear Sulfhydryl-BIND Stripwell Microplate Individually Wrapped w/out Lid Nonsterile

2510 PK50
EUR 847.02

Phospho Antibody Stripping Solution

AKR-102 1 kit
EUR 470.4
Description: This reagent allows you to strip phospho antibodies from protein blots and subsequently re-probe the same blot.

Nitrocellulose Stripping Buffer 10X

10750015-1 250 mL
EUR 34.22

Nitrocellulose Stripping Buffer 10X

10750015-2 500 mL
EUR 60.54

Progesterone ELISA Kit (1 Strip Plate)

K025-H1 1x96 well plate
EUR 300

Progesterone ELISA Kit (5 Strip Plate)

K025-H5 5x96 well plate
EUR 1132

Testosterone ELISA Kit (1 Strip Plate)

K032-H1 1x96 well plate
EUR 312

Testosterone ELISA Kit (5 Strip Plate)

K032-H5 5x96 well plate
EUR 1174

Testosterone ELISA Kit, (1 Strip Plate)

K080-H1 1x96 well plate
EUR 371

Testosterone ELISA Kit, (5 Strip Plate)

K080-H5 5x96 well plate
EUR 1480

Western blot recycling stripping buffer, 50 ml (10X)

90101 50 ml
EUR 285.6

Nylon Membrane Stripping Buffer 10X, Sterile

10750016-1 250 mL
EUR 26.54
Description: Tris/EDTA/Denhardts (TE+Denhardts)

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